Università degli Studi di Milano, Department of Veterinary Science for Health, Animal Production and Food Safety, Via Celoria 10, 20133 Milan, Italy.
Res Vet Sci. 2013 Apr;94(2):372-7. doi: 10.1016/j.rvsc.2012.08.005. Epub 2012 Sep 3.
The application of Kit Radicaux Libres (KRL) test to assess total blood antioxidant activity in pigs was evaluated. The KRL has been validated and is widely used in humans for assessing the effectiveness of natural or pharmaceutical treatments, and in vitro to evaluate the antioxidant activities of natural or synthetic antioxidants. In this study the sensitivity of the KRL test in assessing the effectiveness of dietary antioxidant supplementation (vitamin E and plant extract) was evaluated in two different phases of pig breeding. The first trial, in post-weaned piglets (40 piglets/group) fed dietary vitamin E supplementation for 60 days, indicated that there was a higher total antioxidant activity (P=0.032) of whole blood and of red blood cells (P=0.001) than for control pigs. The second trial indicated that long-term supplementation of water soluble plant extract (20 pigs/group) from the leaves of Verbenaceae (Lippia spp.) tended (P=0.091) to increase antioxidant activity in the whole blood of treated, rather than control pigs. These results indicate that the KRL might be recommended as one of efficient means for evaluating antioxidant activity of dietary ingredients fed to pigs.
自由基清除能力（KRL）测试在评估猪全血总抗氧化活性中的应用。该方法已在人类中得到验证并广泛应用，用于评估天然或药物治疗的有效性，以及体外评估天然或合成抗氧化剂的抗氧化活性。在这项研究中，评估了 KRL 测试在评估不同阶段猪饲养中饮食抗氧化补充剂（维生素 E 和植物提取物）有效性的敏感性。第一阶段试验，在断奶仔猪（每组 40 头）中进行了 60 天的维生素 E 饮食补充，结果表明全血和红细胞的总抗氧化活性（P=0.032）以及红细胞的总抗氧化活性（P=0.001）高于对照组。第二阶段试验表明，长期补充从马鞭草科（ Lippia spp.）叶子中提取的水溶性植物提取物（每组 20 头），处理组而不是对照组猪的全血抗氧化活性呈上升趋势（P=0.091）。这些结果表明，KRL 可作为评估猪日粮中抗氧化成分的有效方法之一。
