Department of Pharmaceutical and Biomedical Sciences, Università degli Studi di Salerno, Fisciano (SA), Italy.
J Agric Food Chem. 2013 Feb 27;61(8):1686-93. doi: 10.1021/jf302815t. Epub 2012 Sep 17.
The phytochemical profile of a hydroalcoholic extract of Citrus aurantium var. amara L. peel, used as herbal medicine, was characterized by HPLC-PDA-MS. Two di-C-glycosyl flavones (vincenin II and diosmetin 6,8-di-C-glucoside), a series of flavones (luteolin 7-O-neohesperidoside, rhoifolin, and neodiosmin), and flavanone (neoeriocitrin, naringin, and neohesperidin) 7-O-neohesperidosides and two methoxyflavones (nobiletin and tangeretin), commonly present in Citrus, were identified. Furthermore, brutieridin and melitidin, two 3-hydroxy-3-methylglutaryl flavanone glycosides, were also characterized along with rhoifolin 4'-glucoside and three coumarins (8,3'-β-D-glucopyranosyloxy-2'-hydroxy-3'-methylbutyl-7-methoxycoumarin, merazin hydrate, and isomerazin). A preparative isolation procedure followed by NMR spectroscopy confirmed the proposed structures of the major flavonoids and identified the coumarins. The phenolic content was found to be 14.8 mg mL(-1), and naringin and neohesperidin were the compounds present in the highest concentration (3.6 and 2.6 mg mL(-1)). The extract of C. aurantium peel inhibited significantly (p < 0.05) both histamine- and dextran-induced edema in rats in a concentration-dependent manner (IC(50) = 119.6 and 118.3 mg kg(-1), respectively), providing evidence for the therapeutic use of C. aurantium var. amara peel.
柑橘属芸香科植物酸橙皮的水醇提取物，用作草药，其植物化学成分通过 HPLC-PDA-MS 进行了特征描述。两种二-C-糖苷黄酮（柠檬素二糖苷 II 和二香叶素 6,8-二-C-葡萄糖苷）、一系列黄酮类化合物（木樨草素 7-O-新橙皮糖苷、圣草酚和新橙皮苷）、黄烷酮（新橙皮苷、柚皮苷和新橙皮苷 7-O-新橙皮糖苷）和两种甲氧基黄酮（诺米林和桔皮素），这些都是柑橘属植物中常见的成分。此外，还鉴定出两种 3-羟基-3-甲基戊二酰基黄酮糖苷，布屈里丁和梅利汀，以及圣草酚 4'-葡萄糖苷和三种香豆素（8,3'-β-D-吡喃葡萄糖苷氧基-2'-羟基-3'-甲基丁基-7-甲氧基香豆素、水合美迪紫檀素和异美迪紫檀素）。通过制备分离程序和 NMR 光谱对其进行了结构确认，这些主要黄酮类化合物和香豆素的结构得到了鉴定。该提取物的酚类含量为 14.8mg mL(-1)，柚皮苷和新橙皮苷的含量最高（3.6 和 2.6mg mL(-1)）。该提取物以浓度依赖的方式显著抑制（p < 0.05）组胺和葡聚糖诱导的大鼠水肿（IC(50)分别为 119.6 和 118.3mg kg(-1)），为酸橙皮的治疗用途提供了证据。
