Institute of Sciences of Food Production, National Research Council, 07100 Sassari, Italy.
Department of Biomedical Sciences, University of Cagliari, 09100 Cagliari, Italy.
Molecules. 2020 Jul 13;25(14):3186. doi: 10.3390/molecules25143186.
Pompia is a Citrus species belonging to Sardinian endemic biodiversity. Health benefits were attributed to its flavedo rind extracts and essential oils while the juice qualities have never been investigated. In this paper, the antioxidant, antimicrobial, and other biological properties of Pompia juice were studied. A combined LCMS/electrochemical/biological approach was used to clarify a still debated phylogeny of this species and to explain the role of its juice phenolic compounds. A closer phylogenetic relationship with lemon and citron, rather than oranges was suggested. Sensors-based electrochemical measures, together with LCMS qualitative and quantitative analyses, revealed a high contribution of ascorbic acid and phenolics with low redox potential, isorhamnetin 3--rutinoside, diosmin, and diosmetin 6,8-diglucoside, to antioxidant capacity. The biological assays demonstrated a marked effect of low concentration of Pompia juice against reactive oxygen species (ROS) starting from 50 µg mL, and a moderate capacity to reduce ROS damages on cell membrane. Treatments with Pompia juice also resulted in a significant reduction (20%) of the metabolic activity of SW48 colon cancer cells. Lastly, MIC, MBC, and MBIC antimicrobial assays demonstrated that Pompia and lemon juices have inhibitory and antibiofilm effects against the pathogenic bacteria , , and .
蓬皮亚是一种柑橘属植物，属于撒丁岛特有生物多样性。其果皮提取物和精油具有健康益处，而果汁的品质从未被研究过。本文研究了蓬皮亚果汁的抗氧化、抗菌和其他生物特性。采用 LCMS/电化学/生物学综合方法，阐明了该物种仍有争议的系统发育，并解释了其果汁酚类化合物的作用。研究表明，与橙子相比，它与柠檬和香橼的亲缘关系更为密切。基于传感器的电化学测量方法，结合 LCMS 定性和定量分析，揭示了其果汁中高浓度的抗坏血酸和低氧化还原电位的酚类化合物，如异鼠李素 3--芸香糖苷、橙皮苷、香叶木素 6,8-二葡萄糖苷，具有很强的抗氧化能力。生物测定表明，蓬皮亚果汁在 50μg mL 的低浓度下对活性氧（ROS）有显著的作用，并且对细胞膜的 ROS 损伤有一定的还原能力。蓬皮亚果汁处理还显著降低了 SW48 结肠癌细胞的代谢活性（20%）。最后，MIC、MBC 和 MBIC 抗菌试验表明，蓬皮亚和柠檬果汁对 、 和 等病原菌具有抑制和抗生物膜作用。
