Lewton Jaron R, Woodward Adrienne D, Moser Ronny L, Thelen Kyan M, Moeser Adam J, Trottier Nathalie L, Tempelman Robert J, Rozeboom Dale W
Department of Animal Science, Michigan State University, East Lansing, MI 48824, USA.
United Animal Health, Sheridan, IN 46069, USA.
Transl Anim Sci. 2022 Jun 20;6(3):txac083. doi: 10.1093/tas/txac083. eCollection 2022 Jul.
The objective of this experiment was to evaluate the effects of a multi-strain -based direct-fed microbial (DFM) on nursery pig health as indicated by intestinal mucosal and blood plasma immunological markers and intestinal morphology. Eighty pigs, of equal number of barrows and gilts (initial BW: 7.0 ± 0.60 kg), weaned at 21 ± 1 d of age were randomly allotted to sixteen pens, with five pigs per pen. Two dietary treatments were implemented, a basal control (CON) and a basal control plus DFM (CDFM). Both diets were corn, soybean meal, and distillers dried grains based and were formulated to meet or exceed all nutritional requirements (NRC, 2012) and manufactured on site. Diets were fed for 42 d. On d 21 and 42 of the experiment, one pig per pen was randomly selected and euthanized, with equal number of males and females represented. Blood samples were collected prior to euthanasia for assessment of plasma concentrations of immunoglobulin A (IgA) and intestinal fatty acid binding protein. Segments of the gastrointestinal tract including duodenum, jejunum, ileum, ascending and distal colon were removed for analysis of intestinal morphology, and levels of interleukin 6, interleukin 10 (IL-10), and tumor necrosis factor alpha. Jejunal villus height was greater in the CDFM pigs as compared with CON pigs ( 0.02) and ascending colon crypt depth tended to be greater on d 21 ( 0.10). Compared to CON, CDFM significantly increased overall plasma IgA ( 0.03) (0.58 vs. 0.73 0.05 mg/mL, respectively), while it tended to increase plasma IgA ( 0.06) on d 21 (0.34 vs. 0.54 ± 0.07 mg/mL, respectively) and tended to increase overall IL-10 ( 0.10) in the jejunum (113 vs. 195 ± 35 pg/mL, respectively). Addition of a multi-strain -based DFM may have an early benefit to nursery pig health status, observed through specific changes in morphology and both systemic and localized immunological markers.
本实验的目的是通过肠道黏膜和血浆免疫标志物以及肠道形态来评估一种基于多菌株的直接投喂微生物（DFM）对保育猪健康的影响。80头猪（公猪和母猪数量相等，初始体重：7.0±0.60千克），在21±1日龄断奶，随机分配到16个栏中，每栏5头猪。实施了两种日粮处理，一种是基础对照（CON），另一种是基础对照加DFM（CDFM）。两种日粮均以玉米、豆粕和干酒糟为基础，其配方满足或超过所有营养需求（NRC，2012）并在现场生产。日粮饲喂42天。在实验的第21天和第42天，每栏随机选择1头猪进行安乐死，公母数量相等。在安乐死之前采集血样，以评估血浆中免疫球蛋白A（IgA）和肠道脂肪酸结合蛋白的浓度。取出包括十二指肠、空肠、回肠、升结肠和远段结肠在内的胃肠道段，用于分析肠道形态以及白细胞介素6、白细胞介素10（IL-10）和肿瘤坏死因子α的水平。与CON组猪相比，CDFM组猪的空肠绒毛高度更高（P<0.02），并且在第21天升结肠隐窝深度有增加的趋势（P<0.10）。与CON组相比，CDFM组显著提高了血浆总体IgA水平（P<0.03）（分别为0.58和0.73±0.05毫克/毫升），而在第21天血浆IgA有增加的趋势（P<0.06）（分别为0.34和0.54±0.07毫克/毫升），并且空肠中总体IL-10有增加的趋势（P<0.10）（分别为113和195±35皮克/毫升）。通过形态学以及全身和局部免疫标志物的特定变化观察到，添加基于多菌株的DFM可能对保育猪的健康状况有早期益处。
