Yang Yuyin, Wang Jie, Liao Jingqiu, Xie Shuguang, Huang Yi
State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China.
Microb Ecol. 2014 Nov;68(4):785-93. doi: 10.1007/s00248-014-0457-7. Epub 2014 Jul 10.
Polycyclic aromatic hydrocarbons (PAHs) are one of the major pollutants in soils in oil exploring areas. Biodegradation is the major process for natural elimination of PAHs from contaminated soils. Functional genes can be used as biomarkers to assess the biodegradation potential of indigenous microbial populations. However, little is known about the distribution of PAH-degrading genes in the environment. The links between environmental parameters and the distribution of PAH metabolic genes remain essentially unclear. The present study investigated the abundance and diversity of naphthalene dioxygenase genes in the oil-contaminated soils in the Shengli Oil Field (China). Spatial variations in the density and diversity of naphthalene dioxygenase genes occurred in this area. Four different sequence genotypes were observed in the contaminated soils, with the predominance of novel PAH-degrading genes. Pearson's correlation analysis illustrated that gene abundance had positive correlations with the levels of total organic carbon and aromatic hydrocarbons, while gene diversity showed a negative correlation with the level of polar aromatics. This work could provide some new insights toward the distribution of PAH metabolic genes and PAH biodegradation potential in oil-contaminated ecosystems.
多环芳烃（PAHs）是石油勘探区域土壤中的主要污染物之一。生物降解是受污染土壤中PAHs自然消除的主要过程。功能基因可作为生物标志物来评估本地微生物种群的生物降解潜力。然而，关于PAH降解基因在环境中的分布情况知之甚少。环境参数与PAH代谢基因分布之间的联系仍基本不清楚。本研究调查了中国胜利油田石油污染土壤中萘双加氧酶基因的丰度和多样性。该区域萘双加氧酶基因的密度和多样性存在空间变异。在受污染土壤中观察到四种不同的序列基因型，其中新型PAH降解基因占主导地位。Pearson相关性分析表明，基因丰度与总有机碳和芳烃水平呈正相关，而基因多样性与极性芳烃水平呈负相关。这项工作可为石油污染生态系统中PAH代谢基因的分布和PAH生物降解潜力提供一些新的见解。
