The price of tags in protein localization studies.
作者信息
Margolin William
机构信息
Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, Houston, Texas, USA.
出版信息
J Bacteriol. 2012 Dec;194(23):6369-71. doi: 10.1128/JB.01640-12. Epub 2012 Sep 7.
Abstract
摘要
相似文献
1
The price of tags in protein localization studies.
J Bacteriol. 2012 Dec;194(23):6369-71. doi: 10.1128/JB.01640-12. Epub 2012 Sep 7.
2
The helical MreB cytoskeleton in Escherichia coli MC1000/pLE7 is an artifact of the N-Terminal yellow fluorescent protein tag.
J Bacteriol. 2012 Dec;194(23):6382-6. doi: 10.1128/JB.00505-12. Epub 2012 Aug 17.
3
Protein localization in Escherichia coli cells: comparison of the cytoplasmic membrane proteins ProP, LacY, ProW, AqpZ, MscS, and MscL.
J Bacteriol. 2010 Feb;192(4):912-24. doi: 10.1128/JB.00967-09. Epub 2009 Dec 11.
4
Thiol-yne radical reaction mediated site-specific protein labeling via genetic incorporation of an alkynyl-L-lysine analogue.
Org Biomol Chem. 2013 Apr 28;11(16):2624-9. doi: 10.1039/c3ob27116a.
5
Matrix-assisted laser desorption ionization-time of flight mass spectrometry assigns Escherichia coli to the phylogroups A, B1, B2 and D.
Int J Med Microbiol. 2014 Nov;304(8):977-83. doi: 10.1016/j.ijmm.2014.06.004. Epub 2014 Jul 7.
6
Comparison of three methods to study biofilm formation by clinical strains of Escherichia coli.
Diagn Microbiol Infect Dis. 2013 Mar;75(3):252-5. doi: 10.1016/j.diagmicrobio.2012.11.019. Epub 2013 Jan 11.
7
Sortase-mediated fluorescent labeling of CRISPR complexes.
Methods Enzymol. 2019;616:43-59. doi: 10.1016/bs.mie.2018.10.031. Epub 2018 Dec 17.
8
Quantification of very low-abundant proteins in bacteria using the HaloTag and epi-fluorescence microscopy.
Sci Rep. 2019 May 27;9(1):7902. doi: 10.1038/s41598-019-44278-0.
9
[Bacterial culture methods for staining flagellae].
Vet Med Nauki. 1974;11(8):78-83.
10
Development and evaluation of a dot blot assay for rapid determination of invasion-associated gene ibeA directly in fresh bacteria cultures of E. coli.
Folia Microbiol (Praha). 2012 Nov;57(6):557-61. doi: 10.1007/s12223-012-0171-0. Epub 2012 Jun 7.
引用本文的文献
1
C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerin.
Elife. 2025 Aug 28;14:RP105937. doi: 10.7554/eLife.105937.
2
C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerin.
bioRxiv. 2025 Jan 20:2025.01.19.633811. doi: 10.1101/2025.01.19.633811.
3
Protein degradation kinetics measured by microinjection and live-cell fluorescence microscopy.
Sci Rep. 2024 Nov 7;14(1):27153. doi: 10.1038/s41598-024-76224-0.
4
Hypothesis: bacteria live on the edge of phase transitions with a cell cycle regulated by a water-clock.
Theory Biosci. 2024 Nov;143(4):253-277. doi: 10.1007/s12064-024-00427-2. Epub 2024 Nov 6.
5
A vector system for single and tandem expression of cloned genes and multi-colour fluorescent tagging in .
Microbiology (Reading). 2024 May;170(5). doi: 10.1099/mic.0.001461.
6
Versatile Labeling and Detection of Endogenous Proteins Using Tag-Assisted Split Enzyme Complementation.
ACS Chem Biol. 2021 Apr 16;16(4):671-681. doi: 10.1021/acschembio.0c00925. Epub 2021 Mar 18.
7
Strategies for Site-Specific Labeling of Receptor Proteins on the Surfaces of Living Cells by Using Genetically Encoded Peptide Tags.
Chembiochem. 2021 May 14;22(10):1717-1732. doi: 10.1002/cbic.202000797. Epub 2021 Feb 26.
8
Supramolecular assembly of the LdcI upon acid stress.
Proc Natl Acad Sci U S A. 2021 Jan 12;118(2). doi: 10.1073/pnas.2014383118.
9
Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.
Microbiol Mol Biol Rev. 2020 Oct 28;84(4). doi: 10.1128/MMBR.00008-20. Print 2020 Nov 18.
10
Hypothesis: nucleoid-associated proteins segregate with a parental DNA strand to generate coherent phenotypic diversity.
Theory Biosci. 2021 Feb;140(1):17-25. doi: 10.1007/s12064-020-00323-5. Epub 2020 Oct 23.
本文引用的文献
1
The helical MreB cytoskeleton in Escherichia coli MC1000/pLE7 is an artifact of the N-Terminal yellow fluorescent protein tag.
J Bacteriol. 2012 Dec;194(23):6382-6. doi: 10.1128/JB.00505-12. Epub 2012 Aug 17.
2
Subcellular localization of RNA and proteins in prokaryotes.
Trends Genet. 2012 Jul;28(7):314-22. doi: 10.1016/j.tig.2012.03.008. Epub 2012 Apr 21.
3
Segregation of molecules at cell division reveals native protein localization.
Nat Methods. 2012 Apr 8;9(5):480-2. doi: 10.1038/nmeth.1955.
4
FtsA forms actin-like protofilaments.
EMBO J. 2012 May 16;31(10):2249-60. doi: 10.1038/emboj.2012.76. Epub 2012 Mar 30.
5
Image analysis in fluorescence microscopy: bacterial dynamics as a case study.
Bioessays. 2012 May;34(5):427-36. doi: 10.1002/bies.201100148. Epub 2012 Mar 13.
6
Helical insertion of peptidoglycan produces chiral ordering of the bacterial cell wall.
Proc Natl Acad Sci U S A. 2012 Mar 6;109(10):E595-604. doi: 10.1073/pnas.1117132109. Epub 2012 Feb 17.
7
Extreme C terminus of bacterial cytoskeletal protein FtsZ plays fundamental role in assembly independent of modulatory proteins.
J Biol Chem. 2012 Mar 30;287(14):10945-57. doi: 10.1074/jbc.M111.330324. Epub 2012 Feb 1.
8
The bacterial actin MreB rotates, and rotation depends on cell-wall assembly.
Proc Natl Acad Sci U S A. 2011 Sep 20;108(38):15822-7. doi: 10.1073/pnas.1108999108. Epub 2011 Sep 8.
9
Bacterial cell wall: thinking globally, actin locally.
Curr Biol. 2011 Aug 23;21(16):R628-30. doi: 10.1016/j.cub.2011.06.056.
10
Direct membrane binding by bacterial actin MreB.
Mol Cell. 2011 Aug 5;43(3):478-87. doi: 10.1016/j.molcel.2011.07.008.
Zotero 插件