C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV 26506, USA.
Electrophoresis. 2012 Sep;33(17):2674-81. doi: 10.1002/elps.201200173.
Aqueous phospholipid preparations comprised of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) are prevalent materials for biological characterization and become gel-like near physiological temperature, but have a low viscosity below 24°C. The rheology of 20% phospholipid preparations of [DMPC]/[DHPC] = 2.5 reveals that, under conditions utilized for fluid steering, the materials are shear-thinning power-law fluids with a power-law index ranging from 0.30 through 0.90. Phospholipid preparations are utilized to steer fluids in microfluidic chips and support hydrodynamic delivery of sample across a double T injection region in a chip. The fact that the phospholipids are fully integrated as a valving material as well as a separation medium is demonstrated through the separation of linear oligosaccharides labeled with 1-aminopyrene-3,6,8-trisulfonic acid.
由 1,2-二肉豆蔻酰-sn-甘油-3-磷酸胆碱（DMPC）和 1,2-二己酰基-sn-甘油-3-磷酸胆碱（DHPC）组成的水性磷脂制剂是用于生物学特性表征的常用材料，在接近生理温度时呈凝胶状，但在 24°C 以下时粘度较低。[DMPC]/[DHPC] = 2.5 的 20%磷脂制剂的流变学表明，在用于流体转向的条件下，这些材料是剪切稀化幂律流体，幂律指数范围从 0.30 到 0.90。磷脂制剂用于在微流控芯片中引导流体，并支持样品在芯片中的双 T 注入区进行水力输送。通过用 1-氨基蒽-3,6,8-三磺酸标记的线性寡糖的分离证明了磷脂作为阀材料以及分离介质的完全集成。
