Departament de Ciènces Médiques Bàsiques, Unitat de Microbiologia, IISPV, Universitat Rovira i Virgili, Reus, Spain.
FEMS Microbiol Lett. 2012 Dec;337(1):48-54. doi: 10.1111/1574-6968.12003. Epub 2012 Oct 3.
Bacteria of the genus Aeromonas are found worldwide in aquatic environments and may produce human infections. In 2010, two new clinical species, Aeromonas sanarellii and Aeromonas taiwanensis, were described on the basis of one strain recovered from wounds of hospitalized patients in Taiwan. So far, only four environmental isolates of A. sanarellii and one of A. taiwanensis have been recorded from waste water in Portugal and an additional clinical strain of A. taiwanensis from the faeces of a patient with diarrhoea in Israel. In the present study, strains belonging to these two species were identified from chironomid egg masses from the same area in Israel by sequencing the rpoD gene. This represents a new environmental habitat for these novel species. The first data on the virulence genes and antibiotic susceptibility are provided. The isolates of these two new species possess multiple virulence genes and are sensitive to amikacin, aztreonam, cefepime, cefoxatime, ceftazidime, ciprofloxacin, gentamicin, piperacillin-tazobactam, tigecycline, tobramycin, trimethoprim-sulfamethoxazole and imipenem. The key phenotypic tests for the differentiation of these new species from their closest relative Aeromonas caviae included the utilization of citrate, growth at 45 °C in sheep blood agar and acid production of cellobiose.
气单胞菌属的细菌广泛存在于水生环境中，可能导致人类感染。2010 年，基于从台湾住院患者伤口中分离到的一株菌，描述了两种新的临床种，即杀鲑气单胞菌和台湾气单胞菌。迄今为止，仅从葡萄牙的废水中记录到了 4 株杀鲑气单胞菌的环境分离株和 1 株台湾气单胞菌的环境分离株，还有一株来自以色列腹泻患者粪便中的台湾气单胞菌临床分离株。在本研究中，通过对来自以色列同一地区摇蚊卵块的 rpoD 基因进行测序，鉴定出这两个种的菌株。这代表了这些新型物种的一个新的环境栖息地。首次提供了关于毒力基因和抗生素敏感性的相关数据。这些两个新种的分离株具有多种毒力基因，对阿米卡星、氨曲南、头孢吡肟、头孢噻肟、头孢他啶、环丙沙星、庆大霉素、哌拉西林他唑巴坦、替加环素、妥布霉素、复方新诺明和亚胺培南敏感。这些新种与其最接近的亲缘种嗜水气单胞菌的关键表型试验包括利用柠檬酸盐、在绵羊血琼脂上 45°C 生长和纤维二糖产酸。
