Sela Rotem, Laviad-Shitrit Sivan, Halpern Malka
Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, University of Haifa, Haifa, Israel.
Department of Biology and Environment, Faculty of Natural Sciences, University of Haifa, Tivon, Israel.
Front Microbiol. 2020 Nov 6;11:586678. doi: 10.3389/fmicb.2020.586678. eCollection 2020.
Chironomids (; ), also known as non-biting midges, are one of the most abundant insects in freshwater habitats. Our aim was to understand whether the metamorphosis developmental stages affect the endogenous microbiota composition of . Toward our objective, we analyzed the endogenous microbiota composition of ' four life stages: egg masses, larvae, pupae, and adults. Significant differences were found between the microbiota compositions of the different developmental stages of this species. We observed a decline in bacterial diversity as the insect evolved from egg mass to adult, while the highest richness was observed in the pupal stage. Although there were significant differences between the microbiota compositions of each life stage, a bacterial core, which included 27 Amplicon Sequence Variants (ASVs), was found in all the developmental life stages (in ≥75% of samples). Chironomids are natural reservoirs of and species, and the and ASVs were part of the core bacteria. The presence of the gene, which is specific to , confirmed the presence of this species in all the chironomid's life stages. Thus, the results provide important insights about the host-microbe interactions in chironomids with a specific understanding of chironomids--microbiota interactions.
摇蚊（; ），也被称为非吸血蠓，是淡水生境中数量最多的昆虫之一。我们的目的是了解变态发育阶段是否会影响摇蚊的内生微生物群组成。为实现这一目标，我们分析了摇蚊四个生命阶段（卵块、幼虫、蛹和成虫）的内生微生物群组成。在该摇蚊物种的不同发育阶段的微生物群组成之间发现了显著差异。我们观察到，随着昆虫从卵块发育到成虫，细菌多样性下降，而在蛹期观察到最高的丰富度。尽管每个生命阶段的微生物群组成存在显著差异，但在所有发育生命阶段（≥75%的样本）中都发现了一个细菌核心，其中包括27个扩增子序列变体（ASV）。摇蚊是 和 物种的天然宿主， 和 ASV是核心细菌的一部分。 基因的存在，该基因是 特有的，证实了该物种在摇蚊所有生命阶段的存在。因此，这些结果为摇蚊中的宿主-微生物相互作用提供了重要见解，特别是对摇蚊-微生物群相互作用有了具体的认识。
