Laboratório de Virologia Aplicada, Departamento de Microbiologia, Imunologia e Parasitologia, Centro de Ciências Biológicas, UFSC, Florianópolis, Brazil.
J Appl Microbiol. 2012 Dec;113(6):1554-63. doi: 10.1111/jam.12010. Epub 2012 Oct 4.
To evaluate the stability in seawater of human adenovirus (HAdV2), murine norovirus (MNV-1) and hepatitis A virus (HAV) in a shellfish depuration system with and without ultraviolet (UV) treatment.
Seawater was seeded with viruses and disinfected using a 36 W lamp. Samples were collected at 24, 48, 72, 96 and 120 h; viruses were concentrated and the viral decay was evaluated using molecular and cell culture methods. Based on the molecular results, at 120 h of disinfection, there was a reduction of more than 3 log(10) for HAdV2 and HAV; MNV-1, a 4.5 log(10) reduction was observed at 72 h. Infectious MNV-1 was not detected after 72 h of treatment; while HAdV2 remained infectious. Seawater not treated demonstrated a progressive viral reduction for the three viruses tested.
The UV reduced the number of viral particles, and the results indicate there is natural and gradual decrease of viral load and viability in seawater.
UV irradiation is the method of choice for shellfish depuration in many countries; this work showed useful information about the viral stability in seawater and application of UV to water disinfection to be used in shellfish depuration tanks.
评估贝类净化系统中紫外线（UV）处理前后，人腺病毒（HAdV2）、鼠诺如病毒（MNV-1）和甲型肝炎病毒（HAV）在海水中的稳定性。
用病毒对海水进行接种并用 36 W 灯进行消毒。在 24、48、72、96 和 120 h 收集样品；使用分子和细胞培养方法浓缩病毒并评估病毒衰减。基于分子结果，在消毒 120 h 时，HAdV2 和 HAV 的减少量超过 3 个对数（10）；MNV-1 在 72 h 时观察到 4.5 个对数（10）的减少。处理 72 h 后未检测到感染性 MNV-1；而 HAdV2 仍具有传染性。未处理的海水对三种测试病毒均表现出逐渐减少的病毒数量。
UV 减少了病毒颗粒的数量，结果表明海水中的病毒载量和活力自然且逐渐下降。
紫外线照射是许多国家贝类净化的首选方法；这项工作提供了有关病毒在海水中稳定性以及紫外线在贝类净化水箱中用于水消毒的应用的有用信息。
