Department of Basic Medical Sciences, Laboratory of Biology, School of Medicine, University of Athens, 11527 Athens, Greece.
World J Gastroenterol. 2012 Aug 28;18(32):4419-26. doi: 10.3748/wjg.v18.i32.4419.
To detect of colorectal cancer (CRC) circulating tumour cells (CTCs) surface antigens, we present an assay incorporating cadmium selenide quantum dots (QDs) in these paper.
The principle of the assay is the immunomagnetic separation of CTCs from body fluids in conjunction with QDs, using specific antibody biomarkers: epithelial cell adhesion molecule antibody, and monoclonal cytokeratin 19 antibody. The detection signal was acquired from the fluorescence signal of QDs. For the evaluation of the performance, the method under study was used to isolate the human colon adenocarcinoma cell line (DLD-1) and CTCs from CRC patients' peripheral blood.
The minimum detection limit of the assay was defined to 10 DLD-1 CRC cells/mL as fluorescence was measured with a spectrofluorometer. Fluorescence-activated cell sorting analysis and Real Time RT-PCR, they both have also been used to evaluate the performance of the described method. In conclusion, we developed a simple, sensitive, efficient and of lower cost (than the existing ones) method for the detection of CRC CTCs in human samples. We have accomplished these results by using magnetic bead isolation and subsequent QD fluorescence detection.
The method described here can be easily adjusted for any other protein target of either the CTC or the host.
为了检测结直肠癌（CRC）循环肿瘤细胞（CTC）的表面抗原，我们提出了一种在这些纸张中结合硒化镉量子点（QDs）的检测方法。
该检测方法的原理是结合 QDs 利用特异性抗体生物标志物，即上皮细胞黏附分子抗体和单克隆角蛋白 19 抗体进行体液中 CTC 的免疫磁分离。检测信号来自 QDs 的荧光信号。为了评估性能，该方法用于分离人结肠腺癌细胞系（DLD-1）和 CRC 患者外周血中的 CTCs。
该检测方法的最小检测限定义为 10 个 DLD-1 CRC 细胞/mL，因为使用分光荧光计测量荧光。荧光激活细胞分选分析和实时 RT-PCR 也被用于评估所描述方法的性能。总之，我们开发了一种简单、敏感、高效且成本更低（低于现有方法）的方法，用于检测人样本中的 CRC CTCs。我们通过使用磁珠分离和随后的 QD 荧光检测来实现这些结果。
这里描述的方法可以很容易地调整用于 CTC 或宿主的任何其他蛋白靶标。
