Cai Huabo, Yuan Zhanggen, Fei Qing, Zhao Jiayao
Department of ICU, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016;
Exp Ther Med. 2012 Mar;3(3):433-436. doi: 10.3892/etm.2011.426. Epub 2011 Dec 20.
Elderly patients face the problems of morbidity and mortality due to age-mediated disabilities. The purpose of the present study was to investigate the expression of thrombospondin-1 (TSP-1) and transforming growth factor-β (TGF-β) in aging mice, and its probable mechanism in the pathological changes of aging myocardium. The aging model group (AM) comprised 30-month-old mice, while the control group comprised 2-month-old mice. The pathological changes were explored by H&E staining, and the contents of superoxide dismulase (SOD) and malondialdehyde (MDA) in the hearts were determined by xanthine oxidation or TBA colorimetry. TSP-1 and TGF-β expression in the left ventricular myocardium was also measured by immunohistochemistry. The results showed that the activities of SOD decreased and the MDA content increased markedly in the hearts of the AM group compared to the control group. H&E staining showed that the control group myocardial cells lined up in order with clear structure and stained equably, while the AM group myocardial cells lined up in disorder with an augmented cell body and the appearance of many granules and interstitial fibrosis. Compared to the control group, in the hearts of the AM group, TSP-1 and TGF-β protein expression in myocardial cells showed a significant increase (P<0.01). TSP-1 and TGF-β expression increased in the myocardium, which may be related to pathological changes of age-related heart diseases, such as hypertrophy, fibrosis of myocardial cells and microvessel dissepiment thickening.
老年患者由于年龄介导的残疾而面临发病和死亡问题。本研究的目的是探讨血小板反应蛋白-1（TSP-1）和转化生长因子-β（TGF-β）在衰老小鼠中的表达及其在衰老心肌病理变化中的可能机制。衰老模型组（AM）由30月龄小鼠组成，而对照组由2月龄小鼠组成。通过苏木精-伊红（H&E）染色探索病理变化，通过黄嘌呤氧化法或硫代巴比妥酸（TBA）比色法测定心脏中超氧化物歧化酶（SOD）和丙二醛（MDA）的含量。还通过免疫组织化学测量左心室心肌中TSP-1和TGF-β的表达。结果显示，与对照组相比，AM组心脏中SOD活性降低，MDA含量显著增加。H&E染色显示，对照组心肌细胞排列有序，结构清晰，染色均匀，而AM组心肌细胞排列紊乱，细胞体增大，出现许多颗粒和间质纤维化。与对照组相比，AM组心脏心肌细胞中TSP-1和TGF-β蛋白表达显著增加（P<0.01）。心肌中TSP-1和TGF-β表达增加，这可能与年龄相关性心脏病的病理变化有关，如心肌细胞肥大、纤维化和微血管分隔增厚。
