Barber D, Carpizo J, Garcia-Rumbao M C, Polo F, Juan F
Alergia e Immunologia Abelló, S.A. C/Miguel Fleta, Madrid, Spain.
Ann Allergy. 1990 Jan;64(1):43-6.
Marked in vitro allergenic potency variations were observed among six different olive pollen batches as determined by RAST inhibition, in direct correlation with the content of a multimeric component of 55,000 D as analyzed by size-exclusion high performance liquid chromotography. Electrophoretical patterns displayed differences in a heterogeneous band of 20,000 D and isoelectric point around 5, which probably represents the monomeric form of the major allergen.
通过放射性变应原吸附试验(RAST)抑制法测定发现,六个不同批次的橄榄花粉在体外的致敏效力存在显著差异,这与通过尺寸排阻高效液相色谱分析的55,000 D多聚体成分含量直接相关。电泳图谱显示,在20,000 D的异质条带以及等电点约为5处存在差异,这可能代表主要过敏原的单体形式。