Leibniz Institute for Natural Product Research and Infection Biology, HKI, Beutenbergstr. 11a, D-07745, Jena, Germany.
Org Biomol Chem. 2012 Dec 21;10(47):9338-43. doi: 10.1039/c2ob26296g. Epub 2012 Sep 12.
A genome mining study led to the identification of a previously unrecognised siderophore biosynthesis gene cluster in the nitrogen-fixing bacterium Cupriavidus taiwanensis LMG19424. Based upon predicted structural residues, a convenient strategy for an NMR-assisted isolation of the associated metabolite was designed. The structure of the purified siderophore, taiwachelin, was fully characterized by spectroscopic methods and chemical derivatisation.
一项基于基因组挖掘的研究在固氮菌 Cupriavidus taiwanensis LMG19424 中鉴定到一个先前未被识别的铁载体生物合成基因簇。基于预测的结构残基，设计了一种用于通过 NMR 辅助分离相关代谢物的便捷策略。通过光谱方法和化学衍生化，对纯化的铁载体 taiwachelin 的结构进行了全面表征。
