Department of Chemistry, Via della Lastruccia, 3-50019, Sesto Fiorentino, Florence, Italy.
J Mass Spectrom. 2012 Sep;47(9):1207-20. doi: 10.1002/jms.3030.
High-performance liquid chromatography coupled with ion spray mass spectrometry in the tandem mode with both negative and positive ionization was used for investigating a variety of polyphenolic compounds in four genotypes of Fragaria vesca berries. About 60 phenolic compounds belonging to the compound classes of phenolic acids, ellagitannins, ellagic acid derivatives, flavonols, monomeric and oligomeric flavanols, dihydrochalcones and anthocyanins were reported, providing for the first time a quite complete picture of polyphenolic composition of F. vesca berries. Some of the polyphenols herein investigated, such as a tris-galloyl-hexahydroxydiphenoyl-hexose, two castalagin/vescalagin-like isomers and peonidin-malonylglucoside, were described for the first time. Principal component analysis applied on original HPLC-MS/MS data, acquired in multiple reaction monitoring mode, successfully discriminated the four investigated cultivars on the basis of their polyphenolic composition, highlighting the fundamental role of mass spectrometry for food characterization.
采用高效液相色谱-串联离子喷雾质谱联用技术（正负离子模式），对四种草莓属弗吉尼亚草莓品种中的多种多酚化合物进行了研究。共鉴定出约 60 种酚类化合物，属于酚酸类、鞣花单宁类、鞣花酸衍生物类、黄酮醇类、单体和二聚黄烷醇类、二氢查耳酮类和花色苷类，首次全面描述了弗吉尼亚草莓果实的多酚组成。本文还首次描述了一些多酚类化合物，如三没食子酰基六羟二苯酰基六葡萄糖、两种拟栗木鞣花丹宁/韦司卡林类似物和矢车菊素-丙二酰葡萄糖苷。基于原始 HPLC-MS/MS 数据（多重反应监测模式采集）进行的主成分分析，成功地根据多酚组成对四种供试品种进行了区分，突出了质谱法在食品特性分析中的重要作用。
