实验室规模下从疏果桃果实中提取多酚的条件具有抗氧化、降血糖和降血脂特性。

Lab Scale Extracted Conditions of Polyphenols from Thinned Peach Fruit Have Antioxidant, Hypoglycemic, and Hypolipidemic Properties.

作者信息

Dai Kun, Wei Yingying, Jiang Shu, Xu Feng, Wang Hongfei, Zhang Xin, Shao Xingfeng

机构信息

Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, China.

出版信息

Foods. 2021 Dec 31;11(1):99. doi: 10.3390/foods11010099.

Thinned peach polyphenols (TPPs) were extracted by ultrasonic disruption and purified using macroporous resin. Optimized extraction conditions resulted in a TPPs yield of 1.59 ± 0.02 mg GAE/g FW, and optimized purification conditions resulted in a purity of 43.86% with NKA-9 resin. TPPs composition was analyzed by UPLC-ESI-QTOF-MS/MS; chlorogenic acid, catechin, and neochlorogenic acid were the most abundant compounds in thinned peaches. Purified TPPs exhibited scavenging activity on DPPH, ABTS, hydroxyl radical, and FRAP. TPPs inhibited α-amylase and α-glucosidase by competitive and noncompetitive reversible inhibition, respectively. TPPs also exhibited a higher binding capacity for bile acids than cholestyramine. In summary, TPPs from thinned peaches are potentially valuable because of their high antioxidant, hypoglycemic, and hypolipidemic capacities, and present a new incentive for the comprehensive utilization of thinned peach fruit.

采用超声破碎法提取疏果桃多酚（TPPs），并用大孔树脂进行纯化。优化后的提取条件下TPPs得率为1.59±0.02 mg GAE/g FW，优化后的纯化条件下使用NKA - 9树脂纯度达到43.86%。通过超高效液相色谱 - 电喷雾电离 - 四极杆飞行时间串联质谱（UPLC - ESI - QTOF - MS/MS）分析TPPs的组成；绿原酸、儿茶素和新绿原酸是疏果桃中含量最丰富的化合物。纯化后的TPPs对二苯基苦味酰基自由基（DPPH）、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）、羟基自由基和铁离子还原抗氧化能力（FRAP）均具有清除活性。TPPs分别通过竞争性和非竞争性可逆抑制作用抑制α-淀粉酶和α-葡萄糖苷酶。TPPs对胆汁酸的结合能力也高于消胆胺。综上所述，疏果桃中的TPPs因其高抗氧化、降血糖和降血脂能力而具有潜在价值，为疏果桃果实的综合利用提供了新的契机。