直接计数亚基揭示了人类甘氨酸受体的化学计量。
Stoichiometry of the human glycine receptor revealed by direct subunit counting.
机构信息
Institut de Ciències Fotòniques, 08860 Castelldefels (Barcelona), Spain, Institut universitaire en santé mentale de Québec, Québec, G1J 2G3 Canada.
出版信息
J Neurosci. 2012 Sep 12;32(37):12915-20. doi: 10.1523/JNEUROSCI.2050-12.2012.
Abstract
The subunit stoichiometry of heteromeric glycine-gated channels determines fundamental properties of these key inhibitory neurotransmitter receptors; however, the ratio of α1- to β-subunits per receptor remains controversial. We used single-molecule imaging and stepwise photobleaching in Xenopus oocytes to directly determine the subunit stoichiometry of a glycine receptor to be 3α1:2β. This approach allowed us to determine the receptor stoichiometry in mixed populations consisting of both heteromeric and homomeric channels, additionally revealing the quantitative proportions for the two populations.
摘要
同型二聚体甘氨酸门控通道的亚基组成决定了这些关键抑制性神经递质受体的基本特性；然而，每个受体的α1-和β-亚基的比例仍存在争议。我们使用 Xenopus 卵母细胞中的单分子成像和逐步光漂白直接确定甘氨酸受体的亚基组成比为 3α1:2β。这种方法使我们能够在由异型和同型通道组成的混合群体中确定受体的组成比，此外还揭示了这两种群体的定量比例。
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