基于 PCR 的土耳其蜜蜂气管螨（Acarapis woodi）检测。
PCR-based detection of the honeybee tracheal mite (Acarapis woodi) in Türkiye.
机构信息
T.C. Ministry of Agriculture and Forestry, Samsun Veterinary Control Institute, Honeybee Diseases Laboratory, Samsun, Türkiye.
Faculty of Agriculture, Department of Plant Protection, Kırşehir Ahi Evran University, Kırşehir, 40100, Türkiye.
出版信息
Parasitol Res. 2023 Jul;122(7):1663-1670. doi: 10.1007/s00436-023-07871-x. Epub 2023 May 13.
Acarapis woodi (Rennie 1921) (Acari: Tarsonemidae) is one of the mites that settles in the respiratory system of honeybees (Apis mellifera L. (Hymenoptera, Apidae)) and distributed throughout the world. It causes significant economic losses on honey production. In Türkiye, studies on the existence of A. woodi are very limited and so far, no studies on the molecular diagnosis and phylogenetic of it have been reported in Türkiye. This study was conducted to investigate the prevalence of A. woodi in Türkiye, especially in areas where beekeeping is intense. Diagnosis of A. woodi was performed using both microscopic and molecular methods using specific PCR primers. Adult honeybee samples were collected from 1.193 hives in 40 provinces of Türkiye between 2018 and 2019. Based on identification studies, the presence of A. woodi was detected in a total of 3 hives (0.5%) in 2018 and 4 hives (0.7%) in 2019. This is the first report for determination of A. woodi in Türkiye.
伍德氏副蜱（Rennie 1921）（蜱螨目：跗线螨科）是一种定居在蜜蜂（Apis mellifera L.（膜翅目，蜜蜂科））呼吸系统中的螨虫，分布于世界各地。它会对蜂蜜生产造成重大的经济损失。在土耳其，关于 A. woodi 的存在的研究非常有限，到目前为止，在土耳其还没有关于其分子诊断和系统发育的研究报告。本研究旨在调查土耳其 A. woodi 的流行情况，特别是在养蜂业密集的地区。使用特定的 PCR 引物，通过显微镜和分子方法对 A. woodi 进行诊断。从 2018 年至 2019 年，在土耳其的 40 个省份的 1193 个蜂箱中收集成年蜜蜂样本。基于鉴定研究，在 2018 年共发现 3 个蜂箱（0.5%）和 2019 年 4 个蜂箱（0.7%）存在 A. woodi。这是在土耳其首次确定 A. woodi 的存在。
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