White S I, Friedmann P S, Moss C, Simpson J M
Br J Dermatol. 1986 Dec;115(6):663-8. doi: 10.1111/j.1365-2133.1986.tb06646.x.
We have investigated the contributions of dose per unit area and area of application to the degree of sensitivity induced by dinitrochlorobenzene (DNCB). Subjects sensitized with 35.4 micrograms/cm2 showed equal sensitization whether the total dose was 250 micrograms applied to 7.1 cm2 or 62.5 micrograms applied to 1.8 cm2. Three groups of subjects were sensitized with 16.4 micrograms/cm2 applied as 56 micrograms on 3.5 cm2, 116 micrograms on 7.1 cm2 or 232 micrograms on 14.2 cm2. Although the total dose changed by a factor of four, the proportions of subjects sensitized were comparable (66, 50 and 55% respectively), and their degrees of reactivity were similar. This contrasts with previous findings that when the area of sensitization was fixed and the concentration was increased over a similar range, highly significant increases in the proportion of sensitized subjects and degree of reactivity occurred.
我们研究了单位面积剂量和涂抹面积对二硝基氯苯（DNCB）诱导的敏感程度的影响。用35.4微克/平方厘米致敏的受试者，无论总剂量是250微克涂抹于7.1平方厘米，还是62.5微克涂抹于1.8平方厘米，致敏程度均相同。三组受试者分别用16.4微克/平方厘米的剂量，即56微克涂抹于3.5平方厘米、116微克涂抹于7.1平方厘米或232微克涂抹于14.2平方厘米进行致敏。尽管总剂量变化了四倍，但致敏受试者的比例相当（分别为66%、50%和55%），且他们的反应程度相似。这与之前的研究结果形成对比，之前的研究发现，当致敏面积固定且浓度在类似范围内增加时，致敏受试者的比例和反应程度会显著增加。
