Instituto de Investigaciones en Catálisis y Petroquímica, Facultad de Ingeniería Química, Universidad Nacional del Litoral, Provincia de Santa Fe, Argentina.
Bioresour Technol. 2012 Nov;123:727-32. doi: 10.1016/j.biortech.2012.08.035. Epub 2012 Aug 19.
A revalorization of discarded carrots as substrate for the production of second-generation ethanol is proposed. In order to increase the fermentable sugar concentration of the musts two strategies were studied: Strategy 1 consisted in the enzymatic hydrolysis of bagasse must and Strategy 2 by which carrots were milled, dropped into distilled water and hydrolyzed with different enzymes prior to compressing and filtering to obtain carrot must. By applying Strategy 2 using 0.05% (v/v) of the enzyme Optimase CX255 at 70°C and pH 5.5 during 2.5h, the fermentable sugars extracted increased 3.5 times. In this way, the production of 77.5L of ethanol for each ton of discarded carrots was achieved. This process yielded bagasse as byproduct, which could be used for animal feed.
提出了将废弃胡萝卜重新利用为生产第二代乙醇的底物。为了提高醪液中的可发酵糖浓度,研究了两种策略:策略 1 是利用酶水解蔗渣醪液,策略 2 是将胡萝卜磨碎,放入蒸馏水中,用不同的酶水解,然后压榨和过滤,得到胡萝卜醪液。通过应用策略 2,在 70°C 和 pH5.5 条件下,使用 0.05%(v/v)的Optimase CX255 酶,水解 2.5 小时,可提取的发酵糖增加了 3.5 倍。通过这种方式,每吨废弃胡萝卜可生产 77.5 升乙醇。该过程产生的蔗渣副产品可用于动物饲料。