Center for Industry, University and Government Cooperation, Nagasaki University, Nagasaki, Japan.
Biol Pharm Bull. 2012;35(9):1413-6. doi: 10.1248/bpb.b212011.
Recent advances in quantitative approaches to laser desorption/ionization mass spectrometry (LDI-MS) are selectively reviewed. Numerous efforts have been made to use matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and related techniques for rapid screening and/or monitoring of biological events. Among the various mass ionization spectrometric analysis techniques, MALDI-MS has exceptional potential for high-throughput analysis. Although quantitative analysis with MALDI-MS is challenging, the expansion of its utility is inevitable. It is notable that practical improvement of MALDI-MS can be achieved by preparation of an uniform matrix using binary matrix systems. Broad applications have been established with a self-assembled monolayer for MALDI-MS, referred to as SAMDI-MS. The method could be an essential tool not only for rapid screening but also as a sensitive probe for surface sciences. Complementary to these approaches, labeling of molecules for LDI-MS is introduced as potential tool for the selective detection of specific target molecules.
本文选择性地综述了激光解吸/电离质谱（LDI-MS）定量方法的最新进展。人们已经做出了许多努力，利用基质辅助激光解吸/电离质谱（MALDI-MS）和相关技术来快速筛选和/或监测生物事件。在各种质谱离子化分析技术中，MALDI-MS 具有高通量分析的巨大潜力。尽管 MALDI-MS 的定量分析具有挑战性，但它的应用范围的扩大是不可避免的。值得注意的是，通过使用二元基质系统制备均匀的基质，可以实现 MALDI-MS 的实际改进。自组装单层用于 MALDI-MS 的广泛应用，被称为 SAMDI-MS。该方法不仅可以作为快速筛选的重要工具，而且可以作为表面科学的灵敏探针。作为这些方法的补充，用于 LDI-MS 的分子标记被引入作为选择性检测特定靶分子的潜在工具。
