Wang Poguang, Giese Roger W
Department of Pharmaceutical Sciences and Barnett Institute, Bouve College, Northeastern University, Boston, MA 02115, USA.
Department of Pharmaceutical Sciences and Barnett Institute, Bouve College, Northeastern University, Boston, MA 02115, USA.
J Chromatogr A. 2017 Feb 24;1486:35-41. doi: 10.1016/j.chroma.2017.01.040. Epub 2017 Jan 18.
Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) has been used for quantitative analysis of small molecules for many years. It is usually preceded by an LC separation step when complex samples are tested. With the development several years ago of "modern MALDI" (automation, high repetition laser, high resolution peaks), the ease of use and performance of MALDI as a quantitative technique greatly increased. This review focuses on practical aspects of modern MALDI for quantitation of small molecules conducted in an ordinary way (no special reagents, devices or techniques for the spotting step of MALDI), and includes our ordinary, preferred methods The review is organized as 18 recommendations with accompanying explanations, criticisms and exceptions.
基质辅助激光解吸电离质谱（MALDI-MS）多年来一直用于小分子的定量分析。在测试复杂样品时，通常在液相色谱分离步骤之前使用。随着几年前“现代MALDI”（自动化、高重复频率激光、高分辨率峰）的发展，MALDI作为一种定量技术的易用性和性能大大提高。本综述重点关注以普通方式（MALDI点样步骤无需特殊试剂、设备或技术）进行小分子定量的现代MALDI的实际应用方面，包括我们常用的首选方法。该综述按照18条建议进行组织，并附有解释、批评意见和例外情况。
