Department of Ophthalmology, University of Cologne, Cologne, Germany.
TICEBA GmbH, Im Neuenheimer Feld 517, Heidelberg, Germany.
Sci Rep. 2018 Aug 22;8(1):12564. doi: 10.1038/s41598-018-30021-8.
UVB irradiation has been linked to pathogenesis of pterygium, a conjunctival tumor growing onto transparent cornea, the windscreen of the eye. Due to corneal anatomy, ambient UVB irradiation is amplified at the stem cell-containing nasal limbus. The aim of this study was to analyse the effect of a UV-blocking contact lens (UVBCL, senofilcon A, Class 1 UV blocker) on limbal epithelial cells and fibroblasts under UVB irradiation compared to a non-UVB-blocking contact lens. UVBCL prevented UVB-induced DNA damage (as assessed by cyclobutane pyrimidine dimer immunostaining) as well as a decrease in proliferation and scratch wound closure rate of both limbal epithelial and fibroblast cells. Similarly, UVBCL protected limbal epithelial cells from UVB-induced loss of their phenotype in terms of colony forming efficiency and stem cell marker expression (ABCB5, P63α, integrin β1) compared to controls. Moreover, with UVBCL pro-inflammatory cytokines such as TNFα and MCP1 remained unchanged. These data demonstrate the significance of UV-protection in preserving the limbal niche in response to at least short-term UVB. Our data support the use of UVBCL in protecting limbal niche cells, especially after limbal stem cell transplantation and in patients after pterygium surgery, to help prevent recurrences.
紫外线照射与翼状胬肉的发病机制有关，翼状胬肉是一种生长在透明角膜（眼睛的挡风玻璃）上的结膜肿瘤。由于角膜的解剖结构，环境中的 UVB 照射在含有干细胞的鼻侧角膜缘处被放大。本研究旨在分析与非 UVB 阻断接触镜相比，UVB 阻断接触镜（UVBCL，senofilcon A，1 类 UV 阻断剂）对 UVB 照射下角膜缘上皮细胞和成纤维细胞的影响。UVBCL 可防止 UVB 诱导的 DNA 损伤（通过环丁烷嘧啶二聚体免疫染色评估），以及角膜缘上皮细胞和成纤维细胞增殖和划痕愈合率的降低。同样，与对照组相比，UVBCL 还能保护角膜缘上皮细胞免受 UVB 诱导的表型丧失，表型丧失表现在集落形成效率和干细胞标志物表达（ABCB5、P63α、整合素 β1）上。此外，使用 UVBCL 后，促炎细胞因子 TNFα 和 MCP1 保持不变。这些数据表明，在至少短期 UVB 照射下，UV 保护对于维持角膜缘生态位具有重要意义。我们的数据支持使用 UVBCL 来保护角膜缘细胞，特别是在角膜缘干细胞移植后和翼状胬肉手术后的患者中，以帮助预防复发。
