Sir William Dunn School of Pathology, Oxford University, South Parks Road, Oxford OX1 3RE, UK.
J Cell Sci. 2012 Dec 1;125(Pt 23):5881-6. doi: 10.1242/jcs.113506. Epub 2012 Sep 12.
Cep135/Bld10 is a conserved centriolar protein required for the formation of the central cartwheel, an early intermediate in centriole assembly. Surprisingly, Cep135/Bld10 is not essential for centriole duplication in Drosophila, suggesting either that Cep135/Bld10 is not essential for cartwheel formation, or that the cartwheel is not essential for centriole assembly in flies. Using electron tomography and super-resolution microscopy we show that centrioles can form a cartwheel in the absence of Cep135/Bld10, but centriole width is increased and the cartwheel appears to disassemble over time. Using 3D structured illumination microscopy we show that Cep135/Bld10 is localized to a region between inner (SAS-6, Ana2) and outer (Asl, DSpd-2 and D-PLP) centriolar components, and the localization of all these component is subtly perturbed in the absence of Cep135/Bld10, although the ninefold symmetry of the centriole is maintained. Thus, in flies, Cep135/Bld10 is not essential for cartwheel assembly or for establishing the ninefold symmetry of centrioles; rather, it appears to stabilize the connection between inner and outer centriole components.
Cep135/Bld10 是一种保守的中心粒蛋白，对于中央轮的形成（中心粒组装的早期中间产物）是必需的。令人惊讶的是，Cep135/Bld10 对于果蝇中心粒的复制并非必需，这表明 Cep135/Bld10 对于轮辐的形成不是必需的，或者轮辐对于果蝇中心粒的组装不是必需的。我们通过电子断层扫描和超分辨率显微镜显示，即使没有 Cep135/Bld10，中心粒也可以形成轮辐，但中心粒的宽度会增加，并且轮辐似乎会随着时间的推移而解体。我们通过 3D 结构照明显微镜显示，Cep135/Bld10 定位于内（SAS-6、Ana2）和外（Asl、DSPD-2 和 D-PLP）中心粒成分之间的区域，并且在没有 Cep135/Bld10 的情况下，所有这些成分的定位都会受到微妙的干扰，尽管中心粒的九倍对称性得以维持。因此，在果蝇中，Cep135/Bld10 对于轮辐的组装或建立中心粒的九倍对称性并非必需；相反，它似乎稳定了内和外中心粒成分之间的连接。
