Yu Jun, Zhong Yisheng, Cheng Yu, Shen Xi, Wang Jun, Wei Yiyong
Department of Ophthalmology, and.
Exp Ther Med. 2011 May;2(3):513-516. doi: 10.3892/etm.2011.239. Epub 2011 Mar 21.
The aim of the present study was to examine the expression of glutamine synthetase (GS) in rat retinal Müller cells induced by different levels of hydrostatic pressure using a novel pressure mechanism. pH, PCO(2) or PO2 in culture medium as determined by gas analysis was used to examine the pressure mechanism. GS expression in the Müller cells at different levels of hydrostatic pressure (0, 20, 40, 60 and 80 mmHg/24 h) was examined using immunofluorescence, real-time PCR and Western blotting. There was no significant difference in pH, PCO(2) or PO(2) in the culture medium by gas analysis at the different hydrostatic pressure levels (p>0.05). Immunofluorescence staining showed that GS was expressed in the Müller cells. The expression of GS in the 40 mmHg/24 h and the 60 mmHg/24 h groups was increased significantly compared to that in the 0 mmHg/24 h group (p<0.05). These results suggest that the pressure mechanism which was constructed was effective and that moderate pressure promotes the up-regulation of GS in active Müller cells.
本研究的目的是使用一种新型压力装置，检测不同水平静水压力诱导大鼠视网膜Müller细胞中谷氨酰胺合成酶（GS）的表达。通过气体分析测定培养基中的pH、PCO₂或PO₂，以检测压力装置。使用免疫荧光、实时PCR和蛋白质印迹法检测不同水平静水压力（0、20、40、60和80 mmHg/24 h）下Müller细胞中GS的表达。通过气体分析，不同静水压力水平下培养基中的pH、PCO₂或PO₂无显著差异（p>0.05）。免疫荧光染色显示GS在Müller细胞中表达。与0 mmHg/24 h组相比，40 mmHg/24 h组和60 mmHg/24 h组中GS的表达显著增加（p<0.05）。这些结果表明构建的压力装置是有效的，适度压力促进活性Müller细胞中GS的上调。
