Skurupiy V A, Obedinskaya K S
Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
Bull Exp Biol Med. 2012 Aug;153(4):431-5. doi: 10.1007/s10517-012-1733-5.
Numerical densities of the nuclei were morhometrically evaluated in all myocytes and myocytes expressing nuclear estrogen- and progesterone-receptor complexes, which were revealed immunohistochemically with monoclonal antibodies in C57Bl/6 mice. It was shown that the above quantitative parameters of myometrial cells after the first pregnancy were similar to those in nonpregnant mice by day 10 after delivery. In the third pregnancy, especially developed after the second interrupted pregnancy, proliferation processes in the myometrium were not completed by postpartum day 10, but dramatically progressed. It was associated with a significant decrease in the fraction of myocytes carrying nuclear hormone-receptor complexes with estradiol and progesterone and their disturbed physiological relations in the myometrium during and after pregnancy probably due to dedifferentiation of a considerable part of myocytes.
在C57Bl/6小鼠中，通过单克隆抗体免疫组织化学法显示，对所有心肌细胞以及表达核雌激素和孕激素受体复合物的心肌细胞进行形态计量学评估，以确定细胞核的数量密度。结果表明，首次妊娠后子宫肌层细胞的上述定量参数在分娩后第10天与未怀孕小鼠相似。在第三次妊娠中，尤其是在第二次中断妊娠后出现的情况下，子宫肌层的增殖过程在产后第10天尚未完成，但进展显著。这与携带雌二醇和孕激素核激素受体复合物的心肌细胞比例显著下降以及妊娠期间和产后子宫肌层中它们紊乱的生理关系有关，这可能是由于相当一部分心肌细胞去分化所致。
