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1
Recent contributions in the field of the recombinant expression of disulfide bonded proteins in bacteria.近年来在细菌中重组表达二硫键蛋白领域的进展。
Microb Cell Fact. 2012 Sep 14;11:129. doi: 10.1186/1475-2859-11-129.
2
Pre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli.预先表达巯基氧化酶可显著提高大肠杆菌细胞质中表达的含二硫键的真核蛋白的产量。
Microb Cell Fact. 2011 Jan 7;10:1. doi: 10.1186/1475-2859-10-1.
3
SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm.Shuffle，一种新型大肠杆菌蛋白表达菌株，能够在细胞质中正确折叠二硫键蛋白。
Microb Cell Fact. 2012 May 8;11:56. doi: 10.1186/1475-2859-11-56.
4
Solubility of disulfide-bonded proteins in the cytoplasm of Escherichia coli and its "oxidizing" mutant.二硫键结合蛋白在大肠杆菌细胞质及其“氧化”突变体中的溶解性
World J Gastroenterol. 2005 Feb 21;11(7):1077-82. doi: 10.3748/wjg.v11.i7.1077.
5
Production of disulfide-bonded proteins in Escherichia coli.在大肠杆菌中生产二硫键结合蛋白。
Protein Expr Purif. 2012 Mar;82(1):240-51. doi: 10.1016/j.pep.2011.10.009. Epub 2011 Nov 7.
6
Disruption of reducing pathways is not essential for efficient disulfide bond formation in the cytoplasm of E. coli.在大肠杆菌的细胞质中，还原途径的中断对于高效形成二硫键并非必需。
Microb Cell Fact. 2010 Sep 13;9:67. doi: 10.1186/1475-2859-9-67.
7
Tuned Escherichia coli as a host for the expression of disulfide-rich proteins.经优化的大肠杆菌可作为表达富含二硫键蛋白的宿主。
Biotechnol J. 2011 Jun;6(6):686-99. doi: 10.1002/biot.201000335. Epub 2011 May 13.
8
Unprecedented pathway of reducing equivalents in a diflavin-linked disulfide oxidoreductase.一种双黄素连接的二硫键氧化还原酶中还原当量的空前途径。
Proc Natl Acad Sci U S A. 2017 Nov 28;114(48):12725-12730. doi: 10.1073/pnas.1713698114. Epub 2017 Nov 13.
9
Functional properties of the individual thioredoxin-like domains of protein disulfide isomerase.蛋白质二硫键异构酶各个硫氧还蛋白样结构域的功能特性。
Biochemistry. 1995 Sep 19;34(37):11725-35. doi: 10.1021/bi00037a009.
10
In vivo formation of Cu,Zn superoxide dismutase disulfide bond in Escherichia coli.大肠杆菌中铜锌超氧化物歧化酶二硫键的体内形成
FEBS Lett. 1999 Jan 29;443(3):313-6. doi: 10.1016/s0014-5793(98)01725-6.

引用本文的文献

1
Posttranslational Targeting of a Recombinant Protein Promotes Its Efficient Secretion into the Escherichia coli Periplasm.重组蛋白的翻译后靶向促进其高效分泌到大肠杆菌周质腔中。
Appl Environ Microbiol. 2019 Jun 17;85(13). doi: 10.1128/AEM.00671-19. Print 2019 Jul 1.
2
Optimizing Recombinant Protein Production in the Escherichia coli Periplasm Alleviates Stress.优化大肠杆菌周质腔中重组蛋白的生产可减轻应激。
Appl Environ Microbiol. 2018 May 31;84(12). doi: 10.1128/AEM.00270-18. Print 2018 Jun 15.
3
Directed evolution to improve protein folding in vivo.定向进化提高体内蛋白质折叠。
Curr Opin Struct Biol. 2018 Feb;48:117-123. doi: 10.1016/j.sbi.2017.12.003. Epub 2017 Dec 23.
4
The tunable pReX expression vector enables optimizing the T7-based production of membrane and secretory proteins in E. coli.可调节的 pReX 表达载体能够优化大肠杆菌中基于 T7 的膜蛋白和分泌蛋白的生产。
Microb Cell Fact. 2017 Dec 16;16(1):226. doi: 10.1186/s12934-017-0840-4.
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Recombinant antibody production evolves into multiple options aimed at yielding reagents suitable for application-specific needs.重组抗体生产发展出多种选择，旨在生产出适合特定应用需求的试剂。
Microb Cell Fact. 2015 Sep 2;14:125. doi: 10.1186/s12934-015-0320-7.
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Identification of a heterologous cellulase and its N-terminus that can guide recombinant proteins out of Escherichia coli.鉴定一种能够引导重组蛋白分泌出大肠杆菌的异源纤维素酶及其N端。
Microb Cell Fact. 2015 Apr 10;14:49. doi: 10.1186/s12934-015-0230-8.
7
Soluble overexpression and purification of bioactive human CCL2 in E. coli by maltose-binding protein.利用麦芽糖结合蛋白在大肠杆菌中进行生物活性人CCL2的可溶性过表达及纯化。
Mol Biol Rep. 2015 Mar;42(3):651-63. doi: 10.1007/s11033-014-3812-3. Epub 2014 Nov 13.
8
Bacterial cytoplasm as an effective cell compartment for producing functional VHH-based affinity reagents and Camelidae IgG-like recombinant antibodies.细菌细胞质作为生产基于VHH的功能性亲和试剂和骆驼科IgG样重组抗体的有效细胞区室。
Microb Cell Fact. 2014 Sep 16;13:140. doi: 10.1186/s12934-014-0140-1.
9
High throughput quantitative expression screening and purification applied to recombinant disulfide-rich venom proteins produced in E. coli.高通量定量表达筛选与纯化应用于大肠杆菌中产生的富含二硫键的重组毒液蛋白。
J Vis Exp. 2014 Jul 30(89):e51464. doi: 10.3791/51464.
10
Recombinant polypeptide production in E. coli: towards a rational approach to improve the yields of functional proteins.在大肠杆菌中生产重组多肽：迈向提高功能蛋白产量的合理方法。
Microb Cell Fact. 2013 Nov 1;12:101. doi: 10.1186/1475-2859-12-101.

本文引用的文献

1
An inverse metabolic engineering approach for the design of an improved host platform for over-expression of recombinant proteins in Escherichia coli.一种逆代谢工程方法，用于设计改良的大肠杆菌宿主平台，以过表达重组蛋白。
Microb Cell Fact. 2012 Jul 3;11:93. doi: 10.1186/1475-2859-11-93.
2
A structurally informed autotransporter platform for efficient heterologous protein secretion and display.一种基于结构信息的自转运蛋白平台，用于高效的异源蛋白分泌和展示。
Microb Cell Fact. 2012 Jun 18;11:85. doi: 10.1186/1475-2859-11-85.
3
Selective purification of recombinant neuroactive peptides using the flagellar type III secretion system.利用鞭毛型 III 型分泌系统对重组神经活性肽进行选择性纯化。
mBio. 2012 May 29;3(3). doi: 10.1128/mBio.00115-12. Print 2012.
4
A generalised module for the selective extracellular accumulation of recombinant proteins.一种用于重组蛋白选择性细胞外积累的通用模块。
Microb Cell Fact. 2012 May 28;11:69. doi: 10.1186/1475-2859-11-69.
5
SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm.Shuffle，一种新型大肠杆菌蛋白表达菌株，能够在细胞质中正确折叠二硫键蛋白。
Microb Cell Fact. 2012 May 8;11:56. doi: 10.1186/1475-2859-11-56.
6
Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly.在采采蝇的细菌共生体 S. glossinidius 中表达和细胞外释放具有功能的抗锥虫 Nanobody®。
Microb Cell Fact. 2012 Feb 15;11:23. doi: 10.1186/1475-2859-11-23.
7
IcsA autotransporter passenger promotes increased fusion protein expression on the cell surface.IcsA 自转运载体乘客促进融合蛋白在细胞表面的表达增加。
Microb Cell Fact. 2012 Feb 7;11:20. doi: 10.1186/1475-2859-11-20.
8
Chemical chaperones assist intracellular folding to buffer mutational variations.化学伴侣协助细胞内折叠，缓冲突变变异。
Nat Chem Biol. 2012 Jan 15;8(3):238-45. doi: 10.1038/nchembio.768.
9
Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3).在大肠杆菌 BL21(DE3)中通过α-溶血素分泌系统细胞外过表达重组嗜热毁丝霉角质酶。
Microb Cell Fact. 2012 Jan 12;11:8. doi: 10.1186/1475-2859-11-8.
10
A novel expression system for production of soluble prion proteins in E. coli.一种在大肠杆菌中生产可溶性朊病毒蛋白的新型表达系统。
Microb Cell Fact. 2012 Jan 10;11:6. doi: 10.1186/1475-2859-11-6.

近年来在细菌中重组表达二硫键蛋白领域的进展。

Recent contributions in the field of the recombinant expression of disulfide bonded proteins in bacteria.

出版信息

Microb Cell Fact. 2012 Sep 14;11:129. doi: 10.1186/1475-2859-11-129.

DOI:10.1186/1475-2859-11-129
PMID:22978724
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3462667/
Abstract

The production of heterologous disulfide bonded proteins in bacteria remains a biotechnological challenge. A rapid literature survey results in the identification of some interesting proposals, such as the option of producing functional proteins in the cytoplasm in the presence of sulfhydryl oxidases and isomerases. Furthermore, an ever-increasing number of applications refers to recombinant proteins displayed at the bacterial surface. Time will tell whether these developments will lead to universally accepted laboratory protocols.

摘要

在细菌中生产异源二硫键结合蛋白仍然是一个生物技术挑战。快速的文献调查结果导致了一些有趣的建议的确定，例如在巯基氧化酶和异构酶存在的情况下在细胞质中生产功能性蛋白质的选择。此外，越来越多的应用涉及到在细菌表面展示的重组蛋白。时间将证明这些发展是否会导致普遍接受的实验室方案。