Schleicher E D, Vogt B W
Diabetes Research Group, Institute for Clinical Chemistry, Munich, F.R.G.
Clin Chem. 1990 Jan;36(1):136-9.
We have calibrated a secondary serum protein standard by use (as primary standards) of samples of albumin and polylysine glycated with [14C]glucose in vitro, the glycation of which was assessed by radioactivity measurements and by elementary analysis for C and N. Using this standard for calibration in our improved fructosamine assay, one obtains an average fructosamine value of 247 mumol/L for nondiabetic individuals (or, in terms of total serum protein, 3.2 mumol/g)--about a tenth the value we obtained when we used the fructosamine assay of Johnson et al. (Clin Chim Acta 1983;127:87-95), standardized with desoxymorpholinofructose. In contrast, results corresponded well with the value for mean glycation of serum proteins, 3 mumol/g, determined by a furosine/HPLC method. Evidently the proposed procedure, in which a standard sharing the binding characteristics of endogenous glycated proteins is used together with our modified new fructosamine assay, leads to more realistic values for the concentrations of glycated serum proteins.
我们通过使用体外经[14C]葡萄糖糖基化的白蛋白和聚赖氨酸样本（作为一级标准品）校准了二级血清蛋白标准品，其糖基化通过放射性测量以及碳和氮的元素分析进行评估。在我们改进的果糖胺测定中使用该标准品进行校准，非糖尿病个体的果糖胺平均值得出为247 μmol/L（或者，就总血清蛋白而言为3.2 μmol/g）——约为我们使用Johnson等人（《临床化学学报》1983年；127:87 - 95）用脱氧吗啉果糖校准的果糖胺测定法所得到的值的十分之一。相比之下，结果与通过呋喃素/HPLC方法测定的血清蛋白平均糖基化值3 μmol/g吻合良好。显然，所提出的程序，即使用具有内源性糖基化蛋白结合特性的标准品与我们改进的新果糖胺测定法一起使用，能得出更符合实际的糖基化血清蛋白浓度值。
