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壳聚糖与MTAD去除玷污层的比较扫描电子显微镜评估

A comparative scanning electron microscopy evaluation of smear layer removal with chitosan and MTAD.

作者信息

Zhou H, Li Q, Wei L, Huang S, Zhao S

机构信息

Department of Paediatric Dentistry, School and Hospital of Stomatology, Wenzhou Medical University, P.R. China.

Institue of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, P.R. China.

出版信息

Niger J Clin Pract. 2018 Jan;21(1):76-80. doi: 10.4103/1119-3077.224798.

DOI:10.4103/1119-3077.224798
PMID:29411728
Abstract

UNLABELLED

The aim of the present study was to compare the efficacy of chitosan and MTAD for the smear layer removal from the root canal through a scanning electron microscope (SEM). Thirty teeth were randomly divided into three groups according to the final irrigants: 0.2% chitosan, MTAD, saline (control group). After the mechanical preparation, the samples were irrigated with saline (control group), 0.2% chitosan and MTDA respectively. Then, the samples were split and the smear layer at the apical, middle, and coronal thirds of each root canal was imaged using SEM. The statistical analysis was performed using the Kruskal-Wallis test and the Mann-Whitney U test (α = 5%). The difference between chitosan and MTDA was statistically significant in the apical region (p < 0.05), no significant difference was obtained in the coronal and middle regions in these two experiment groups (p > 0.05). The control group exhibited the lowest efficacy in smear layer removal in all regions. Thus, from the result of the present study, we may conclude that chitosan was more effective in smear layer removal than MTAD especially in the apical third.

CONTEXT

Irrigation, which serves a variety of purposes including antibacterial action, tissue dissolution, cleaning and chelating, plays a centric role in the final success of root canal treatment. Thus, more and more attention has been put on the improvement and development of various irrigation techniques or systems.

AIMS

The aim of the present study was to compare the efficacy of chitosan and MTAD for the smear layer removal from the root canal through scanning electron microscopy (SEM).

SETTINGS AND DESIGN

Thirty single-canal premolars were instrumented with rotary-files and then, randomly assigned to test groups which were irrigated with chitosan and MTDA, and control group was treated with saline. Thereafter, the efficacy of smear layer removal was evaluated by SEM.

MATERIALS AND METHODS

Thirty teeth were randomly divided into three groups according to the final irrigants: 0.2% chitosan, MTAD, saline (control group). After the mechanical preparation, the samples were irrigated with saline (control group), 0.2% chitosan and MTDA respectively. Then, the samples were split and the smear layer at the apical, middle, and coronal thirds of each root canal was imaged using SEM.

STATISTICAL ANALYSIS USED

Kruskal-Walli test and Mann-Whitney U test Results: The difference between chitosan and MTDA was statistically significant in the apical regions (p < 0.05), no significant difference was obtained in the coronal and middle regions in these two experiment groups (p > 0.05). The control group exhibited the lowest efficacy in smear layer removal in all regions.

CONCLUSION

Thus, from the result of present study, we may conclude that chitosan was more effective in smear layer removal than MTAD, especially in the apical third.

摘要

未标注

本研究的目的是通过扫描电子显微镜(SEM)比较壳聚糖和MTAD去除根管内玷污层的效果。30颗牙齿根据最终冲洗液随机分为三组:0.2%壳聚糖组、MTAD组、生理盐水组(对照组)。机械预备后,样本分别用生理盐水(对照组)、0.2%壳聚糖和MTAD冲洗。然后,将样本劈开,使用SEM对每个根管根尖、中部和冠部三分之一处的玷污层进行成像。采用Kruskal-Wallis检验和Mann-Whitney U检验进行统计分析(α = 5%)。壳聚糖和MTAD在根尖区域的差异具有统计学意义(p < 0.05),这两个实验组在冠部和中部区域未获得显著差异(p > 0.05)。对照组在所有区域去除玷污层的效果最低。因此,根据本研究结果,我们可以得出结论,壳聚糖在去除玷污层方面比MTAD更有效,尤其是在根尖三分之一处。

背景

冲洗在根管治疗的最终成功中起着核心作用,其具有多种作用,包括抗菌作用、组织溶解、清洁和螯合作用。因此,越来越多的关注被放在各种冲洗技术或系统的改进和发展上。

目的

本研究的目的是通过扫描电子显微镜(SEM)比较壳聚糖和MTAD去除根管内玷污层的效果。

设置与设计

30颗单根管前磨牙用旋转锉进行预备,然后随机分配到用壳聚糖和MTAD冲洗的试验组,对照组用生理盐水处理。此后,通过SEM评估去除玷污层的效果。

材料与方法

30颗牙齿根据最终冲洗液随机分为三组:0.2%壳聚糖组、MTAD组、生理盐水组(对照组)。机械预备后,样本分别用生理盐水(对照组)、0.2%壳聚糖和MTAD冲洗。然后,将样本劈开,使用SEM对每个根管根尖、中部和冠部三分之一处的玷污层进行成像。

统计分析方法

Kruskal-Wallis检验和Mann-Whitney U检验 结果:壳聚糖和MTAD在根尖区域的差异具有统计学意义(p < 0.05),这两个实验组在冠部和中部区域未获得显著差异(p > 0.05)。对照组在所有区域去除玷污层的效果最低。

结论

因此,根据本研究结果,我们可以得出结论,壳聚糖在去除玷污层方面比MTAD更有效,尤其是在根尖三分之一处。

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