Department of Food Science and Biotechnology, Dongguk University-Seoul, 26, 3-Ga, Pil-dong, Chung-gu, Seoul 100-715, Republic of Korea.
Food Chem. 2012 Dec 15;135(4):2930-3. doi: 10.1016/j.foodchem.2012.06.111. Epub 2012 Jul 13.
The objective of this study was to establish an analytical method to measure pesticides used to cultivate yuza (Citrus junos Sieb. ex Tanaka) and to analyze pesticide residue levels of yuza and yuza tea samples. Risk assessments were also performed by calculating estimated daily intake (EDI) and acceptable daily intake (ADI). An excellent linear correlation was achieved with coefficient correlation values of 0.9750-0.9999. Percent recoveries were 80.4-109.9% for most pesticides with a <6.9% relative standard deviation (RSD). The limits of quantification for the method were 0.10-0.67 μg/ml. The RSD of intra-day and inter-day variability was <15.3%. Seven pesticides in yuza (n=80) and yuza tea (n=75) were analyzed with the optimized analytical method. Acequinocyl, spirodiclofen and carbendazim were detected in yuza samples in the concentration range of 0.07-0.15 μg/g, 0.11-1.89 μg/g, and 0.03-5.15 μg /g, respectively, whereas chlorpyrifos, prothiofos, phosalone, and deltamethrin were not detected in yuza or yuza tea. The concentrations of acequinocyl, spirodiclofen and carbendazim ranged from 0.18-1.05 μg/g, 0.13-0.29 μg/g, and 0.17-2.36 μg/g, respectively, in yuza tea samples. The percent ratios of EDI to ADI for acequinocyl, spirodiclofen, and carbendazim were 24.6%, 22.7%, and 58.5%, respectively.
本研究旨在建立一种分析方法来测定用于种植柚子（Citrus junos Sieb. ex Tanaka）的农药，并分析柚子和柚子茶样品中的农药残留水平。还通过计算估计每日摄入量（EDI）和可接受每日摄入量（ADI）来进行风险评估。与系数相关值 0.9750-0.9999 相比，获得了极好的线性相关性。对于大多数农药，回收率为 80.4-109.9%，相对标准偏差（RSD）<6.9%。该方法的定量限为 0.10-0.67 μg/ml。日内和日间变异性的 RSD<15.3%。用优化的分析方法分析了 80 个柚子（n=80）和 75 个柚子茶（n=75）中的 7 种农药。在柚子样品中检测到了 acequinocyl、spirodiclofen 和 carbendazim，浓度范围分别为 0.07-0.15 μg/g、0.11-1.89 μg/g 和 0.03-5.15 μg /g，而在柚子或柚子茶中未检测到氯吡硫磷、丙硫磷、 phosalone 和溴氰菊酯。acequinocyl、spirodiclofen 和 carbendazim 在柚子茶样品中的浓度范围分别为 0.18-1.05 μg/g、0.13-0.29 μg/g 和 0.17-2.36 μg/g。acequinocyl、spirodiclofen 和 carbendazim 的 EDI 与 ADI 的比例分别为 24.6%、22.7%和 58.5%。
