基于金/氧化锌薄膜表面等离子体共振的生物传感器免疫分析用于检测人唾液中的糖链抗原 15-3 的研制。
Development of an Au/ZnO thin film surface plasmon resonance-based biosensor immunoassay for the detection of carbohydrate antigen 15-3 in human saliva.
机构信息
Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan.
出版信息
Clin Biochem. 2012 Dec;45(18):1689-93. doi: 10.1016/j.clinbiochem.2012.09.001. Epub 2012 Sep 12.
OBJECTIVE
To evaluate a novel surface plasmon resonance (SPR) system for the direct measurement of tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva.
DESIGN AND METHODS
We measured the presence of the tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva using 2 different surface plasmon resonance (SPR) systems. To compare the sensitivity of an SPR biosensor based on thin-film Au/ZnO and the Biacore SPR system, we prepared CA15-3 samples in saliva and analyzed intensity responses to the samples at various concentrations of CA15-3.
RESULTS
The linear detection range of CA15-3 in human saliva with the SPR system based on thin-film Au/ZnO was 2.5-20 U/mL (the cut-off point in cancer patients is around 4 U/mL). The linear range with the Biacore SPR system was 40-300 U/mL.
CONCLUSIONS
These results show that thin-film Au/ZnO-based SPR systems have higher sensitivity and can be used for measuring the levels of CA15-3 in human saliva without concentrating the samples.
目的
评估一种新型的表面等离子体共振（SPR）系统，用于直接测量人唾液中的肿瘤标志物糖链抗原 15-3（CA15-3）。
设计和方法
我们使用 2 种不同的表面等离子体共振（SPR）系统测量人唾液中肿瘤标志物糖链抗原 15-3（CA15-3）的存在。为了比较基于薄膜 Au/ZnO 的 SPR 生物传感器和 Biacore SPR 系统的灵敏度，我们在唾液中制备了 CA15-3 样品，并分析了不同 CA15-3 浓度下对样品的强度响应。
结果
基于薄膜 Au/ZnO 的 SPR 系统检测人唾液中 CA15-3 的线性检测范围为 2.5-20 U/mL（癌症患者的临界值约为 4 U/mL）。Biacore SPR 系统的线性范围为 40-300 U/mL。
结论
这些结果表明，基于薄膜 Au/ZnO 的 SPR 系统具有更高的灵敏度，可用于测量人唾液中 CA15-3 的水平，而无需浓缩样品。
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