Spt4-Spt5复合物：转录延伸的多面调节因子。
The Spt4-Spt5 complex: a multi-faceted regulator of transcription elongation.
作者信息
Hartzog Grant A, Fu Jianhua
机构信息
Department of MCD Biology, University of California, Santa Cruz, CA 95064, USA.
出版信息
Biochim Biophys Acta. 2013 Jan;1829(1):105-15. doi: 10.1016/j.bbagrm.2012.08.007. Epub 2012 Sep 6.
Abstract
In all domains of life, elongating RNA polymerases require the assistance of accessory factors to maintain their processivity and regulate their rate. Among these elongation factors, the Spt5/NusG factors stand out. Members of this protein family appear to be the only transcription accessory proteins that are universally conserved across all domains of life. In archaea and eukaryotes, Spt5 associates with a second protein, Spt4. In addition to regulating elongation, the eukaryotic Spt4-Spt5 complex appears to couple chromatin modification states and RNA processing to transcription elongation. This review discusses the experimental bases for our current understanding of Spt4-Spt5 function and recent studies that are beginning to elucidate the structure of Spt4-Spt5/RNA polymerase complexes and mechanism of Spt4-Spt5 action. This article is part of a Special Issue entitled: RNA polymerase II Transcript Elongation.
摘要
在生命的所有领域中，延长的RNA聚合酶都需要辅助因子的协助来维持其持续合成能力并调节其速率。在这些延长因子中，Spt5/NusG因子尤为突出。该蛋白家族的成员似乎是唯一在生命的所有领域中普遍保守的转录辅助蛋白。在古细菌和真核生物中，Spt5与另一种蛋白Spt4相关联。除了调节延长过程外，真核生物的Spt4-Spt5复合物似乎还将染色质修饰状态和RNA加工与转录延长联系起来。本综述讨论了我们目前对Spt4-Spt5功能理解的实验依据，以及最近开始阐明Spt4-Spt5/RNA聚合酶复合物结构和Spt4-Spt5作用机制的研究。本文是名为“RNA聚合酶II转录延长”的特刊的一部分。
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