Alikhani Mohammad Yousef, Sedighi Iraj, Zamani Alireza, Aslani Mohammad Mehdi, Sadrosadat Taravat
Hamadan University of Medical Sciences, Department of Microbiology, Hamadan, Iran.
Acta Microbiol Immunol Hung. 2012 Sep;59(3):367-74. doi: 10.1556/AMicr.59.2012.3.7.
Diarrhoeagenic Escherichia coli (DEC) represents a main group of enteric pathogens that cause human diarrhoea. Because it is not simply distinguished from normal flora by simple laboratory methods, modern molecular diagnostic assays are necessary. Although it is neither necessary nor applicable to perform PCR for all patients, it is of many advantages to verify the prevalence of DEC in different areas by this method. Knowing the prevalence of DEC in an area, we can focus on few pathogens and narrow our antimicrobial treatment. The aim of this study is to evaluate the contribution of the different DEC categories in children diarrhoea in the west of Iran.The stool specimens of 251 children with diarrhoea were collected from June to September 2007. Polymerase chain reaction (PCR) was performed to determine the presence of enteropathogenic (EPEC), enterotoxigenic (ETEC), entero-invasive (EIEC), Shiga toxin-producing (STEC) and entero-aggregative (EAEC) strains. ETEC strains were isolated from 13 and EAEC strains from 16 children. STEC was detected in 7 children, and no EIEC was isolated. Finally, EPEC strains were isolated in 41 cases. EAEC and EPEC are the most frequent DECs in children less than 10 years of age in West of Iran.
致泻性大肠杆菌（DEC）是引起人类腹泻的主要肠道病原体群体。由于无法通过简单的实验室方法将其与正常菌群简单区分开来，因此现代分子诊断检测方法是必要的。尽管对所有患者进行PCR既不必要也不适用，但通过这种方法验证不同地区DEC的流行情况具有诸多优势。了解一个地区DEC的流行情况，我们可以聚焦于少数病原体并缩小抗菌治疗范围。本研究的目的是评估不同类型的DEC对伊朗西部儿童腹泻的影响。2007年6月至9月收集了251例腹泻儿童的粪便标本。采用聚合酶链反应（PCR）检测肠道致病性（EPEC）、产肠毒素性（ETEC）、肠侵袭性（EIEC）、产志贺毒素性（STEC）和肠集聚性（EAEC）菌株的存在情况。从13名儿童中分离出ETEC菌株，从16名儿童中分离出EAEC菌株。在7名儿童中检测到STEC，未分离出EIEC。最后，在41例中分离出EPEC菌株。EAEC和EPEC是伊朗西部10岁以下儿童中最常见的DEC类型。
