哺乳动物核膜核苷三磷酸酶在核糖核蛋白核质运输中的重要性。
Importance of mammalian nuclear-envelope nucleoside triphosphatase in nucleo-cytoplasmic transport of ribonucleoproteins.
作者信息
Agutter P S, McCaldin B, McArdle H J
出版信息
Biochem J. 1979 Sep 15;182(3):811-9. doi: 10.1042/bj1820811.
Abstract
The nucleoside triphosphate-stimulated efflux of RNA from isolated nuclei was studied under a range of conditions, and the effects of these conditions on the process were compared with the properties of the nucleoside triphosphatase located in the pore complex. A marked similarity between the rate of efflux and the rate of nucleoside triphosphate hydrolysis was apparent, in terms of substrate specificity, sensitivity to treatment with insolubilized trypsin, kinetics and the effects of increased ionic strength and of many inhibitors. These results are taken, in view of earlier evidence, to suggest that the activity of the nucleoside triphosphatase is a prerequisite for nucleo-cytoplasmic RNA transport in vivo. There are some indications that the nuclear-envelope lipid is also involved in regulating the efflux process.
摘要
在一系列条件下研究了核苷三磷酸刺激的RNA从分离细胞核中的流出，并将这些条件对该过程的影响与位于孔复合体中的核苷三磷酸酶的特性进行了比较。就底物特异性、对不溶性胰蛋白酶处理的敏感性、动力学以及离子强度增加和许多抑制剂的影响而言，流出速率与核苷三磷酸水解速率之间存在明显的相似性。鉴于早期的证据，这些结果表明核苷三磷酸酶的活性是体内核质RNA转运的先决条件。有一些迹象表明核膜脂质也参与调节流出过程。
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