Genetics and Molecular Biology Laboratory, Coastal Studies Institute, Bragança Campus, Universidade Federal do Pará, PA, Brazil.
BMC Plant Biol. 2012 Sep 17;12:168. doi: 10.1186/1471-2229-12-168.
Black pepper (Piper nigrum L.) is one of the most popular spices in the world. It is used in cooking and the preservation of food and even has medicinal properties. Losses in production from disease are a major limitation in the culture of this crop. The major diseases are root rot and foot rot, which are results of root infection by Fusarium solani and Phytophtora capsici, respectively. Understanding the molecular interaction between the pathogens and the host's root region is important for obtaining resistant cultivars by biotechnological breeding. Genetic and molecular data for this species, though, are limited. In this paper, RNA-Seq technology has been employed, for the first time, to describe the root transcriptome of black pepper.
The root transcriptome of black pepper was sequenced by the NGS SOLiD platform and assembled using the multiple-k method. Blast2Go and orthoMCL methods were used to annotate 10338 unigenes. The 4472 predicted proteins showed about 52% homology with the Arabidopsis proteome. Two root proteomes identified 615 proteins, which seem to define the plant's root pattern. Simple-sequence repeats were identified that may be useful in studies of genetic diversity and may have applications in biotechnology and ecology.
This dataset of 10338 unigenes is crucially important for the biotechnological breeding of black pepper and the ecogenomics of the Magnoliids, a major group of basal angiosperms.
黑胡椒（Piper nigrum L.）是世界上最受欢迎的香料之一。它被用于烹饪和食物保存，甚至具有药用特性。由疾病引起的生产损失是该作物种植的主要限制因素。主要病害是根腐病和脚腐病，分别是由茄病镰刀菌和辣椒疫霉引起的根部感染所致。了解病原菌与宿主根部之间的分子相互作用对于通过生物技术培育获得抗性品种非常重要。然而，该物种的遗传和分子数据有限。在本文中，首次采用 RNA-Seq 技术描述了黑胡椒的根部转录组。
通过 NGS SOLiD 平台对黑胡椒的根部转录组进行测序，并使用多重 k 方法进行组装。Blast2Go 和 orthoMCL 方法用于注释 10338 个 unigenes。4472 个预测蛋白与拟南芥蛋白质组有约 52%的同源性。两个根蛋白质组鉴定出 615 种蛋白质，这些蛋白质似乎定义了植物的根模式。鉴定出简单序列重复，这可能对遗传多样性的研究有用，并可能在生物技术和生态学中有应用。
这个包含 10338 个 unigenes 的数据集对于黑胡椒的生物技术培育和木兰类植物的生态基因组学至关重要，木兰类植物是一个主要的基生被子植物群。
