嗜热脂肪芽孢杆菌NUB36染色体的遗传图谱。
Genetic map of the Bacillus stearothermophilus NUB36 chromosome.
作者信息
Vallier H, Welker N E
机构信息
Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208.
出版信息
J Bacteriol. 1990 Feb;172(2):793-801. doi: 10.1128/jb.172.2.793-801.1990.
A circular genetic map of Bacillus stearothermophilus NUB36 was constructed by transduction with bacteriophage TP-42C and protoplast fusion. Sixty-four genes were tentatively assigned a cognate Bacillus subtilis gene based on growth response to intermediates or end products of metabolism, cross-feeding, accumulation of intermediates, or their relative order in a linkage group. Although the relative position of many genes on the Bacillus stearothermophilus and Bacillus subtilis genetic map appears to be similar, some differences were detected. The tentative order of the genes in the Bacillus stearothermophilus aro region is aspB-aroBAFEC-tyrA-hisH-(trp), whereas it is aspB-aroE-tyrA-hisH-(trp)-aroHBF in Bacillus subtilis. The aroA, aroC, and aroG genes in Bacillus subtilis are located in another region. The tentative order of genes in the trp operon of Bacillus stearothermophilus is trpFCDABE, whereas it is trpABFCDE in Bacillus subtilis.
通过用噬菌体TP - 42C进行转导和原生质体融合构建了嗜热脂肪芽孢杆菌NUB36的环状遗传图谱。基于对代谢中间产物或终产物的生长反应、交叉饲喂、中间产物积累或它们在连锁群中的相对顺序，64个基因被初步指定了同源的枯草芽孢杆菌基因。尽管嗜热脂肪芽孢杆菌和枯草芽孢杆菌遗传图谱上许多基因的相对位置似乎相似，但也检测到了一些差异。嗜热脂肪芽孢杆菌aro区域中基因的初步顺序是aspB - aroBAFEC - tyrA - hisH - (trp)，而在枯草芽孢杆菌中是aspB - aroE - tyrA - hisH - (trp) - aroHBF。枯草芽孢杆菌中的aroA、aroC和aroG基因位于另一个区域。嗜热脂肪芽孢杆菌trp操纵子中基因的初步顺序是trpFCDABE，而在枯草芽孢杆菌中是trpABFCDE。
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