嗜热脂肪芽孢杆菌中的转导作用。
Transduction in Bacillus stearothermophilus.
作者信息
Welker N E
机构信息
Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208.
出版信息
J Bacteriol. 1988 Aug;170(8):3761-4. doi: 10.1128/jb.170.8.3761-3764.1988.
Abstract
Temperate and virulent bacteriophages isolated from soil were shown to carry out generalized transduction of Bacillus stearothermophilus NUB36. A transducing frequency of 1 X 10(-5) to 7 X 10(-4) was obtained for temperate phages TP-42 and TP-56. The transducing frequency for virulent phage TP-68 was two to three orders of magnitude lower. Cotransfer analysis with the three phages showed that hom-1 is linked to thr-1 and that gly-1 is linked to his-1.
摘要
从土壤中分离出的温和噬菌体和烈性噬菌体可对嗜热脂肪芽孢杆菌NUB36进行普遍性转导。温和噬菌体TP - 42和TP - 56的转导频率为1×10⁻⁵至7×10⁻⁴。烈性噬菌体TP - 68的转导频率低两到三个数量级。对这三种噬菌体进行的共转移分析表明，hom - 1与thr - 1连锁，gly - 1与his - 1连锁。
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