Henríquez R, Blobel G, Aris J P
Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021.
J Biol Chem. 1990 Feb 5;265(4):2209-15.
We have identified the gene for the yeast nucleolar protein p38 and deduced the primary structure of p38 from its sequence. We propose the name NOP1 (nucleolar protein 1) for this gene. NOP1 encodes a 327 amino acid protein of 34,470 daltons and is flanked by potential promoter and polyadenylation sequences. Blot analyses indicate that the mRNA transcribed from NOP1 is approximately 1.3 kilobases in size and that there is one NOP1 gene per haploid genome. The amino-terminal sequence of p38 is homologous with the 31 known amino-terminal residues of the autoimmune antigen fibrillarin, confirming the previously observed similarity between p38 and this mammalian nucleolar protein. Consistent with this, p38 cross-reacts with serum from a patient with the autoimmune disease scleroderma. A putative nuclear localization signal can be identified in p38. Interestingly, a repetitive amino acid sequence motif begins near the amino terminus of p38. This motif is approximately 80 residues long, is rich in glycine and arginine, and shows striking sequence homology to mammalian nucleolins and certain nucleic acid binding proteins.
我们已经鉴定出酵母核仁蛋白p38的基因，并根据其序列推导了p38的一级结构。我们建议将该基因命名为NOP1（核仁蛋白1）。NOP1编码一个由327个氨基酸组成、分子量为34470道尔顿的蛋白质，两侧是潜在的启动子和聚腺苷酸化序列。印迹分析表明，从NOP1转录的mRNA大小约为1.3千碱基，单倍体基因组中存在一个NOP1基因。p38的氨基末端序列与自身免疫抗原原纤维蛋白已知的31个氨基末端残基同源，证实了之前观察到的p38与这种哺乳动物核仁蛋白之间的相似性。与此一致的是，p38与一名患有自身免疫性疾病硬皮病患者的血清发生交叉反应。在p38中可以鉴定出一个假定的核定位信号。有趣的是，一个重复的氨基酸序列基序从p38的氨基末端附近开始。这个基序大约有80个残基长，富含甘氨酸和精氨酸，并且与哺乳动物核仁素和某些核酸结合蛋白具有显著的序列同源性。
