通过酶联免疫吸附测定法检测呼吸道合胞病毒（RSV）特异性免疫球蛋白G、M和A来对儿童呼吸道合胞病毒（RSV）感染进行血清学诊断。
Serodiagnosis of respiratory syncytial virus (RSV) infection in children as measured by detection of RSV-specific immunoglobulins G, M, and A with enzyme-linked immunosorbent assay.
作者信息
Meddens M J, Herbrink P, Lindeman J, van Dijk W C
机构信息
Department of Pathology, Clinical Microbiology and Clinical Immunology, Stichting Samenwerking Delftse Ziekenhuizen, Delft, The Netherlands.
出版信息
J Clin Microbiol. 1990 Jan;28(1):152-5. doi: 10.1128/jcm.28.1.152-155.1990.
Abstract
The diagnostic value of an enzyme-linked immunosorbent assay for detection of respiratory syncytial virus (RSV)-specific immunoglobulin G (IgG), IgM, and IgA in sera from infants and children with proven RSV infection, from a control group, and from patients with symptoms of viral respiratory disease was analyzed. Compared to virus isolation and RSV antigen detection methods, the sensitivity of this assay was 87% and the specificity was 79%. For IgG alone, these were 45 and 92%, for IgM alone they were 48 and 92%, and for IgA alone they were 74 and 95%, respectively.
摘要
分析了酶联免疫吸附测定法在检测确诊为呼吸道合胞病毒（RSV）感染的婴幼儿、对照组以及患有病毒性呼吸道疾病症状患者血清中RSV特异性免疫球蛋白G（IgG）、IgM和IgA的诊断价值。与病毒分离和RSV抗原检测方法相比，该测定法的灵敏度为87%，特异性为79%。单独检测IgG时，灵敏度和特异性分别为45%和92%；单独检测IgM时，分别为48%和92%；单独检测IgA时，分别为74%和95%。
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