Medical Biochemistry and Molecular Biology, Cairo University , Cairo , Egypt.
Immunol Invest. 2012;41(8):864-75. doi: 10.3109/08820139.2012.711407. Epub 2012 Sep 18.
Systemic lupus erythematosus is one of the autoimmune diseases characterized by multisystem involvement associated with autoantibody and immune complex vasculitis along with endothelial cell damage.
to study the possible role of Angiopoietin- 2 (Ang-2) as a recently highlighted inflammatory and angiogenic mediator in the pathogenesis of SLE and its correlation with the state of another inflammatory marker, P-Selectin, as well as with various markers of the disease activity.
The present study included 3 main groups: active SLE patients (group I), inactive SLE patients (group II) and healthy normal control subjects (group III). Groups I and II were subjected to disease activity assessment using the SLEDAI scoring system and measurement of plasma Ang-2 and P-Selectin by ELISA in addition to various laboratory investigations to assess disease activity as: Complete blood count, ESR, serum creatinine, C3, C4 and 24-h urinary proteins.
The mean level of Plasma Ang-2 and P-selectin showed a high significant increase in active group compared to inactive SLE patients and control subjects (p < 0.001).There was a significant positive correlation between Ang-2, P-Selectin, and each of SLEDAI score and 24-h urinary proteins in all SLE patients as well as in the active group, and Ang-2 was a significant independent marker for proteinuria. A significant negative correlation was found between Ang-2, P-Selectin and each of C3, C4. Ang-2 and P-Selectin showed a high sensitivity and specificity in the patients with SLE.
Our study suggests that Ang-2 may be a more useful marker than P-Selectin, C3 and C4 in the assessment of disease activity.
系统性红斑狼疮是一种自身免疫性疾病，其特征为多系统受累，伴有自身抗体和免疫复合物性血管炎以及内皮细胞损伤。
研究血管生成素-2（Ang-2）作为一种新发现的炎症和血管生成介质在系统性红斑狼疮发病机制中的可能作用，及其与另一种炎症标志物 P-选择素以及各种疾病活动标志物的相关性。
本研究包括 3 个主要组：活动期系统性红斑狼疮患者（组 I）、非活动期系统性红斑狼疮患者（组 II）和健康正常对照组（组 III）。使用 SLEDAI 评分系统对组 I 和 II 进行疾病活动评估，并通过 ELISA 测量血浆 Ang-2 和 P-选择素，此外还进行了各种实验室检查以评估疾病活动：全血细胞计数、ESR、血清肌酐、C3、C4 和 24 小时尿蛋白。
与非活动期系统性红斑狼疮患者和对照组相比，活动期组的血浆 Ang-2 和 P-选择素水平显着升高（p<0.001）。在所有系统性红斑狼疮患者以及活动组中，Ang-2、P-选择素与 SLEDAI 评分和 24 小时尿蛋白均呈显着正相关，Ang-2是蛋白尿的独立显着标志物。Ang-2、P-选择素与 C3、C4 呈显着负相关。Ang-2 和 P-选择素在系统性红斑狼疮患者中具有高灵敏度和特异性。
我们的研究表明，在评估疾病活动方面，Ang-2 可能比 P-选择素、C3 和 C4 更有用。
