Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Adv Genet. 2012;79:123-55. doi: 10.1016/B978-0-12-394395-8.00004-9.
The protein microarray technology provides a versatile platform for characterization of hundreds of thousands of proteins in a highly parallel and high-throughput manner. It is viewed as a new tool that overcomes the limitation of DNA microarrays. On the basis of its application, protein microarrays fall into two major classes: analytical and functional protein microarrays. In addition, tissue or cell lysates can also be directly spotted on a slide to form the so-called "reverse-phase" protein microarray. In the last decade, applications of functional protein microarrays in particular have flourished in studying protein function and construction of networks and pathways. In this chapter, we will review the recent advancements in the protein microarray technology, followed by presenting a series of examples to illustrate the power and versatility of protein microarrays in both basic and clinical research. As a powerful technology platform, it would not be surprising if protein microarrays will become one of the leading technologies in proteomic and diagnostic fields in the next decade.
蛋白质微阵列技术提供了一个通用的平台，可用于以高度平行和高通量的方式对数十万种蛋白质进行特征分析。它被视为克服 DNA 微阵列局限性的新工具。基于其应用，蛋白质微阵列分为两类：分析型和功能型蛋白质微阵列。此外，组织或细胞裂解物也可以直接点样到载玻片上，形成所谓的“反相”蛋白质微阵列。在过去的十年中，功能型蛋白质微阵列的应用在研究蛋白质功能以及构建网络和途径方面尤其蓬勃发展。在本章中，我们将回顾蛋白质微阵列技术的最新进展，然后通过一系列示例来说明蛋白质微阵列在基础和临床研究中的强大功能和多功能性。作为一种强大的技术平台，如果蛋白质微阵列在未来十年成为蛋白质组学和诊断领域的领先技术之一，也不会令人感到意外。
