阿萨伊果（Euterpe oleracea）和其他 10 种天然生命染料中的花青素辅助下的后玻璃体脱离和内界膜剥除：尸体眼球的实验研究。

Posterior hyaloid detachment and internal limiting membrane peeling assisted by anthocyanins from acai fruit (Euterpe oleracea) and 10 other natural vital dyes: experimental study in cadaveric eyes.

机构信息

Department of Ophthalmology, Vision Institute (IPEPO), Paulista School of Medicine, Federal University of Sao Paulo, Brazil.

出版信息

Retina. 2013 Jan;33(1):89-96. doi: 10.1097/IAE.0b013e3182618a6d.

DOI:10.1097/IAE.0b013e3182618a6d

PMID:22990318

Abstract

PURPOSE

The purpose of this study was to determine whether natural dyes facilitate posterior hyaloid detachment (posterior vitreous detachment [PVD]) and retinal internal limiting membrane (ILM) peeling in human eyes.

METHODS

Open-sky vitrectomy with posterior hyaloid and ILM removal was performed in 86 human cadaveric eyes. After core vitrectomy, 11 different dyes were injected into the vitreous cavity to aid hyaloid detachment and ILM removal. The dyes were allowed to settle on the macula for 5 minutes after PVD and were removed by mechanical aspiration. Intraocular forceps were used for ILM peeling, which was confirmed by light microscopy of the peeled tissue. Acai fruit (Euterpe oleracea) extract and 10 additional dyes from plants or animal sources were tested: pomegranate (Punica granatum), logwood (Haematoxylum campechianum), chlorophyll extract from alfalfa (Medicago sativa), cochineal (Dactylopius coccus), hibiscus (Hibiscus rosa-sinensis), indigo (Indigofera tinctoria), paprika (Capiscum annuum), turmeric (Curcuma longa), old fustic (Maclura tinctoria), and grape (Vitis vinifera).

RESULTS

The dyes facilitated PVD and ILM peeling. Acai fruit (E. oleracea) extract, logwood (H. campechianum), cochineal (D. coccus), and old fustic (M. tinctoria) facilitated PVD in all cases; dye-assisted PVD was compared with triamcinolone-assisted PVD performed previously in a comparative model. Acai fruit (E. oleracea) extract, cochineal (D. coccus), and chlorophyll extract from alfalfa (M. sativa) showed the best capability for ILM staining; dye-assisted ILM removal was compared with the ILM peeling guided by indocyanine green staining performed previously in a comparative model. Light microscopy confirmed the ILM removal in all cases.

CONCLUSION

Anthocyanin dye of the acai fruit (E. oleracea) and the dyes from cochineal (D. coccus) and chlorophyll extract from alfalfa (M. sativa) resulted in the best capability for posterior hyaloid and ILM staining in human cadaveric eyes and may be a useful tool for vitreoretinal surgery.

摘要

目的

本研究旨在确定天然染料是否有助于人眼的后玻璃体脱离（玻璃体后脱离[PVD]）和视网膜内界膜（ILM）剥离。

方法

对 86 例人尸眼行开放式天空玻璃体切除术联合后玻璃体和 ILM 切除。在核心玻璃体切除术后，将 11 种不同的染料注入玻璃体腔，以辅助玻璃体脱离和 ILM 去除。在 PVD 后，让染料在黄斑区沉淀 5 分钟，然后通过机械抽吸去除。使用眼内镊进行 ILM 剥离，并用剥离组织的光学显微镜进行确认。测试了来自植物或动物来源的巴西莓（Euterpe oleracea）提取物和另外 10 种染料：石榴（Punica granatum）、苏木（Haematoxylum campechianum）、紫花苜蓿（Medicago sativa）中的叶绿素提取物、胭脂虫（Dactylopius coccus）、木槿（Hibiscus rosa-sinensis）、靛蓝（Indigofera tinctoria）、辣椒粉（Capsicum annuum）、姜黄（Curcuma longa）、老黄栌（Maclura tinctoria）和葡萄（Vitis vinifera）。

结果

染料促进了 PVD 和 ILM 剥离。巴西莓（E. oleracea）提取物、苏木（H. campechianum）、胭脂虫（D. coccus）和老黄栌（M. tinctoria）在所有情况下均促进了 PVD；与之前在比较模型中进行的曲安奈德辅助 PVD 相比，对染料辅助 PVD 进行了比较。巴西莓（E. oleracea）提取物、胭脂虫（D. coccus）和紫花苜蓿（M. sativa）中的叶绿素提取物显示出对 ILM 染色的最佳能力；与之前在比较模型中进行的吲哚菁绿染色引导的 ILM 去除相比，对染料辅助 ILM 去除进行了比较。光学显微镜确认了所有情况下的 ILM 去除。