Department of Biomedical Engineering, Inje University, Gimhae, Gyeongnam, Korea.
J Mater Sci Mater Med. 2012 Nov;23(11):2671-8. doi: 10.1007/s10856-012-4738-8. Epub 2012 Sep 19.
Three dimensional tissue engineered scaffolds for the treatment of critical defect have been usually fabricated by salt leaching or gas forming technique. However, it is not easy for cells to penetrate the scaffolds due to the poor interconnectivity of pores. To overcome these current limitations we utilized a rapid prototyping (RP) technique for fabricating tissue engineered scaffolds to treat critical defects. The RP technique resulted in the uniform distribution and systematic connection of pores, which enabled cells to penetrate the scaffold. Two kinds of materials were used. They were poly(ε-caprolactone) (PCL) and poly(D, L-lactic-glycolic acid) (PLGA), where PCL is known to have longer degradation time than PLGA. In vitro tests supported the biocompatibility of the scaffolds. A 12-week animal study involving various examinations of rabbit tibias such as micro-CT and staining showed that both PCL and PLGA resulted in successful bone regeneration. As expected, PLGA degraded faster than PCL, and consequently the tissues generated in the PLGA group were less dense than those in the PCL group. We concluded that slower degradation is preferable in bone tissue engineering, especially when treating critical defects, as mechanical support is needed until full regeneration has occurred.
三维组织工程支架通常采用盐溶或气体形成技术来制备，用于治疗临界缺陷。然而，由于孔的连通性差，细胞不容易穿透支架。为了克服这些当前的局限性，我们利用快速成型（RP）技术来制造组织工程支架，以治疗临界缺陷。RP 技术导致了孔的均匀分布和系统连接，使细胞能够穿透支架。使用了两种材料。它们是聚己内酯（PCL）和聚（D，L-乳酸-乙醇酸）（PLGA），其中 PCL 的降解时间比 PLGA 长。体外试验支持支架的生物相容性。一项为期 12 周的涉及兔子胫骨的各种检查的动物研究，如微 CT 和染色，表明 PCL 和 PLGA 都能成功地再生骨。正如预期的那样，PLGA 的降解速度比 PCL 快，因此 PLGA 组产生的组织比 PCL 组的组织密度小。我们得出结论，在骨组织工程中，较慢的降解是可取的，特别是在治疗临界缺陷时，因为在完全再生发生之前需要机械支撑。
