Zeeman Anne-Marie, der Wel Annemarie Voorberg-van, Kocken Clemens H M
Biomedical Primate Research Centre, Rijswijk, The Netherlands.
Methods Mol Biol. 2013;923:35-49. doi: 10.1007/978-1-62703-026-7_4.
Long-term in vitro cultures of blood-stage parasites are so far feasible only for Plasmodium falciparum and P. knowlesi. In this chapter, we describe short-term ex vivo culturing of P. cynomolgi and P. vivax. We also describe long-term in vitro culturing of P. knowlesi as well as some techniques for synchronizing parasites. Cultured parasites can be used for a variety of purposes, e.g., for in vitro drug assays and antibody-mediated growth inhibition assays.
到目前为止，仅恶性疟原虫和诺氏疟原虫的血液期寄生虫能够进行长期体外培养。在本章中，我们描述了食蟹猴疟原虫和间日疟原虫的短期体外培养。我们还描述了诺氏疟原虫的长期体外培养以及一些寄生虫同步化技术。培养的寄生虫可用于多种目的，例如体外药物测定和抗体介导的生长抑制测定。
