The Department of Surgery, Medical Faculty Mannheim, University Medical Centre Mannheim, Heidelberg University, Heidelberg, Germany.
Can J Surg. 2012 Dec;55(6):389-93. doi: 10.1503/cjs.007911.
Different approaches to study liver regeneration in murine models have been proposed. We investigated the effect of different liver resection models on liver damage and regeneration parameters in mice.
We compared the technical aspect of the 2 most commonly used techniques of 50% and 70% liver resection. Liver damage, as determined by the change in serum alanine aminotransferase and aspartate aminotransferase, as well as the regeneration parameters VEGF and FGF-2 were analyzed at 6 time points. A postoperative vitality score was introduced.
Cholestasis was not observed for either technique. Both resection techniques resulted in full weight recovery of the liver after 240 hours, with no significant difference between sham and resection groups. Postoperative animal morbidity and total protein levels did not differ significantly for either method, indicating early and full functional recovery. However, comparing the mitogenic growth factors FGF-2 and VEGF, a significant increase in serum levels and, therefore, increased growth stimulus, was shown in the extended resection group.
Extended resection led to a greater response in growth factor expression. This finding is important since it shows that growth factor response differs acdording to the extent of resection. We have demonstrated the need to standardize murine hepatic resection models to adequately compare the resulting liver damage.
已经提出了不同的方法来研究鼠模型中的肝再生。我们研究了不同的肝切除模型对小鼠肝损伤和再生参数的影响。
我们比较了两种最常用的 50%和 70%肝切除技术的技术方面。通过检测血清丙氨酸氨基转移酶和天冬氨酸氨基转移酶的变化来分析肝损伤，以及 6 个时间点的血管内皮生长因子和成纤维细胞生长因子-2 等再生参数。引入术后活力评分。
两种技术均未观察到胆汁淤积。两种切除技术均导致肝脏在 240 小时后完全恢复体重，肝切除组与假手术组之间无显著差异。两种方法的术后动物发病率和总蛋白水平均无显著差异，表明早期和完全的功能恢复。然而，比较有丝分裂生长因子 FGF-2 和 VEGF 时，在扩大切除组中显示血清水平显著增加，因此生长刺激增加。
扩大切除导致生长因子表达的反应更大。这一发现很重要，因为它表明生长因子的反应根据切除的范围而不同。我们已经证明需要标准化鼠肝切除模型，以充分比较由此产生的肝损伤。
