Department of Companion and Laboratory Animal Science, Kongju National University, Yesan 340-702, Republic of Korea.
Mol Med Rep. 2012 Dec;6(6):1443-9. doi: 10.3892/mmr.2012.1085. Epub 2012 Sep 17.
The purpose of this study was to determine the effects of baicalein and wogonin, which are compounds derived from the Chinese herb Scutellaria baicalensis, in suppressing the viability of HT-29 human colon cancer cells. Following treatment with baicalein or wogonin, several apoptotic events were observed, including DNA fragmentation, chromatin condensation and increased cell cycle arrest in the G1 phase. Baicalein and wogonin decreased Bcl-2 expression, whereas the expression of Bax was increased in a dose-dependent manner compared with the control. Furthermore, the induction of apoptosis was accompanied by an inactivation of phosphatidylinositol 3-kinase (PI3K)/Akt in a dose-dependent manner. The administration of baicalein to mice resulted in the inhibition of the growth of HT-29 xenografts without any toxicity following 5 weeks of treatment. The results indicated that baicalein induced apoptosis via Akt activation in a p53-dependent manner in the HT-29 colon cancer cells and that it may serve as a chemopreventive or therapeutic agent for HT-29 colon cancer.
本研究旨在探讨黄芩素和汉黄芩素这两种源自中国草药黄芩的化合物对 HT-29 人结肠癌细胞活力的抑制作用。经黄芩素或汉黄芩素处理后，观察到多种细胞凋亡事件，包括 DNA 片段化、染色质浓缩和细胞周期在 G1 期的阻滞增加。与对照组相比，黄芩素和汉黄芩素降低了 Bcl-2 的表达，而 Bax 的表达则呈剂量依赖性增加。此外，细胞凋亡的诱导伴随着磷脂酰肌醇 3-激酶（PI3K）/Akt 的失活，呈剂量依赖性。给予小鼠黄芩素后，在治疗 5 周后未出现任何毒性，即可抑制 HT-29 异种移植物的生长。结果表明，黄芩素通过依赖 p53 的 Akt 激活诱导 HT-29 结肠癌细胞凋亡，可能成为 HT-29 结肠癌的化学预防或治疗剂。
