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16S rRNA 基因测序、基质辅助激光解吸电离飞行时间质谱联用 MALDI Biotyper 和传统表型方法鉴定 α-溶血性链球菌临床分离株：比较。

Identification of clinical isolates of α-hemolytic streptococci by 16S rRNA gene sequencing, matrix-assisted laser desorption ionization-time of flight mass spectrometry using MALDI Biotyper, and conventional phenotypic methods: a comparison.

机构信息

Medical Microbiology and Infectious Diseases, Institute of Life Science, The College of Medicine, Swansea University, Swansea, United Kingdom.

出版信息

J Clin Microbiol. 2012 Dec;50(12):4087-90. doi: 10.1128/JCM.02387-12. Epub 2012 Sep 19.

DOI:10.1128/JCM.02387-12
PMID:22993176
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3502998/
Abstract

Fifty-six α-hemolytic streptococcal isolates were identified using MALDI Biotyper MS (Bruker Daltonics), API 20 Strep (bioMérieux), and BD Phoenix (Becton, Dickinson). The gold standard for identification was 16S rRNA gene sequence analysis with 16S-23S rRNA intergenic spacer sequencing. The following percentages of isolates were correctly identified to the species level: MALDI Biotyper, 46%; BD Phoenix, 35%; and API 20 Strep, 26%.

摘要

采用 MALDI Biotyper MS（布鲁克·道尔顿）、API 20 Strep（生物梅里埃）和 BD Phoenix（贝克顿·迪金森）对 56 株α-溶血性链球菌进行鉴定。鉴定的金标准是 16S rRNA 基因序列分析结合 16S-23S rRNA 基因间隔区测序。以下为各鉴定方法对种水平的正确鉴定百分比：MALDI Biotyper，46%；BD Phoenix，35%；API 20 Strep，26%。

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