Laboratory of Biology and Nano-science, EA4203, Montpellier University 1, 545 av du Pr Viala, 34193 cedex 5, Montpellier, France.
J Biophotonics. 2013 Oct;6(10):765-74. doi: 10.1002/jbio.201200095. Epub 2012 Sep 19.
The goals of this trial were, first, to produce a Raman mapping of decay and sound dentin samples, through accurate analysis of the Raman band spectra variations of mineral and organic components. The second goal was to confirm the correlation between the Raman signal and the signal of a fluorescent camera, by assaying the concentration of pentosidine and natural collagen fluorescent crosslink using reverse phase high-pressure liquid chromatography. The first correlation assumed a possible relationship between the signal observed with the camera and Raman spectroscopy. The second correlation assumed an association with the Maillard reaction. Absence of a correlation for this trial was that no association could be found between Raman spectra characteristics, fluorescence variation and the HPLC assay. Our results void this absence.
首先，通过对矿物质和有机成分的拉曼带光谱变化进行精确分析，生成衰变和有声牙本质样本的拉曼图谱。其次，通过反相高压液相色谱法检测戊糖和天然胶原荧光交联的浓度，确认拉曼信号与荧光相机信号之间的相关性。第一个相关性假设相机和拉曼光谱观察到的信号之间可能存在关系。第二个相关性假设与美拉德反应有关。如果该试验没有相关性，那么拉曼光谱特征、荧光变化和 HPLC 测定之间就没有关联。我们的结果证实了这一点。
