表没食子儿茶素没食子酸酯和鞣花酸在亚硒酸钠诱导的实验性白内障模型中的作用比较
Comparison of the impact of epigallocatechin gallate and ellagic acid in an experimental cataract model induced by sodium selenite.
作者信息
Ergen Irfan, Turgut Burak, Ilhan Nevin
机构信息
Dışkapı YıldırımBeyazıt Training and Research Hospital, Eye Diseases Clinic, Ankara 06330, Turkey.
Department of Ophthalmology, Faculty of Medicine, Firat University, Elazig 23119, Turkey.
出版信息
Int J Ophthalmol. 2017 Apr 18;10(4):499-506. doi: 10.18240/ijo.2017.04.01. eCollection 2017.
AIM
To compare the potential protective effects of epi-gallocatechin gallate (EGCG) and ellagic acid (EA) in an experimental cataract model.
METHODS
Twenty-eight Spraque-Dawley rat pups were assigned into four groups. All the rats, except for those in the control group, were injected subcutaneously sodium selenite to induce experimental cataract on the postpartum ninth day, and between 10 and 14 days. Rats in the sham, EGCG, and EA groups were intraperitoneally administered 50 mg/(kg·d) saline solution, 50 mg/(kg·d) EGCG and 200 mg/(kg·d) EA, respectively. The reduced glutathione (GSH) and malondialdehyde (MDA) levels, total antioxidant status (TAS) and total oxidant status (TOS) in lens supernatants were measured.
RESULTS
The mean cataract gradings in EGCG and EA groups were found to be significantly lower than that in sham group (<0.001). The mean GSH levels and TASs in EGCG and EA groups were significantly higher than that in sham group while mean MDA levels and TOSs in EGCG and EA groups were significantly lower than that in the sham group (<0.001).
CONCLUSION
EGCG and EA have protective effects on cataract development the inhibition of oxidative stress.
目的
比较表没食子儿茶素没食子酸酯（EGCG）和鞣花酸（EA）在实验性白内障模型中的潜在保护作用。
方法
将28只Spraque-Dawley幼鼠分为四组。除对照组外，所有大鼠在出生后第9天以及10至14天皮下注射亚硒酸钠以诱导实验性白内障。假手术组、EGCG组和EA组大鼠分别腹腔注射50 mg/(kg·d)生理盐水溶液、50 mg/(kg·d) EGCG和200 mg/(kg·d) EA。测量晶状体上清液中的还原型谷胱甘肽（GSH）和丙二醛（MDA）水平、总抗氧化状态（TAS）和总氧化状态（TOS）。
结果
发现EGCG组和EA组的平均白内障分级显著低于假手术组（<0.001）。EGCG组和EA组的平均GSH水平和TAS显著高于假手术组，而EGCG组和EA组的平均MDA水平和TOS显著低于假手术组（<0.001）。
结论
EGCG和EA对白内障发展具有保护作用——抑制氧化应激。
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