Svetlov Vladimir, Nudler Evgeny
Department of Biochemistry and Molecular Pharmacology, New York University Langone School of Medicine, New York, NY 10016, USA.
Biochim Biophys Acta. 2013 Jan;1829(1):20-8. doi: 10.1016/j.bbagrm.2012.08.009. Epub 2012 Sep 6.
RNA polymerase II-like enzymes carry out transcription of genomes in Eukaryota, Archaea, and some viruses. They also exhibit fundamental similarity to RNA polymerases from bacteria, chloroplasts, and mitochondria. In this review we take an inventory of recent studies illuminating different steps of basic transcription mechanism, likely common for most multi-subunit RNA polymerases. Through the amalgamation of structural and computational chemistry data we attempt to highlight the most feasible reaction pathway for the two-metal nucleotidyl transfer mechanism, and to evaluate the way catalysis can be linked to translocation in the mechano-chemical cycle catalyzed by RNA polymerase II. This article is part of a Special Issue entitled: RNA polymerase II Transcript Elongation.
RNA聚合酶II样酶在真核生物、古细菌和一些病毒中进行基因组转录。它们也与来自细菌、叶绿体和线粒体的RNA聚合酶表现出基本的相似性。在本综述中，我们总结了近期的研究，这些研究阐明了基本转录机制的不同步骤，这可能是大多数多亚基RNA聚合酶所共有的。通过整合结构和计算化学数据，我们试图突出双金属核苷酸转移机制最可行的反应途径，并评估在RNA聚合酶II催化的机械化学循环中催化作用与易位相联系的方式。本文是名为“RNA聚合酶II转录延伸”的特刊的一部分。
